促进纳他霉素合成的真菌诱导子筛选及诱导条件优化

doi:10.7506/spkx1002-6630-201307041

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 196-200.doi: 10.7506/spkx1002-6630-201307041

促进纳他霉素合成的真菌诱导子筛选及诱导条件优化

魏宝东，潘娅慧，王亚男，王利艳

沈阳农业大学食品学院，辽宁沈阳 110866

收稿日期:2012-05-10 修回日期:2013-02-24 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 魏宝东 E-mail:bdwei2003@yahoo.com.cn
基金资助:
辽宁省教育厅科研项目(L2012248)

Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions

WEI Bao-dong ，PAN Ya-hui，WANG Ya-nan，WANG Li-yan

College of Food Science, Shenyang Agricultural University, Shenyang 110866, China

Received:2012-05-10 Revised:2013-02-24 Online:2013-04-15 Published:2013-03-20
Contact: WEI Bao-dong E-mail:bdwei2003@yahoo.com.cn

摘要/Abstract

摘要： 采用真菌诱导子提高纳他霉素的产量，通过初筛，获得具有正向调节作用的真菌诱导子；采用Box-Behnken试验设计建立数学模型，进行响应面分析优化，确定诱导条件。结果表明：黑曲霉代谢产物诱导子能够有效提高纳他霉素的产量；质量浓度为122μg/mL的黑曲霉代谢产物诱导子在纳他霉素发酵18.3h后加入，诱导78.5h时，发酵液中纳他霉素的发酵产量可达到2.89g/L，为空白对照组的1.68倍。

关键词: 真菌诱导子, 发酵, 纳他霉素, 响应面

Abstract: Preliminary screening was carried out to obtain fungal elicitor that induces natamycin production by Streptomyces chattanovgensis. The optimal induction conditions were established by mathematical modelling mathematical modelling based on Box-Behnken experimental design and response surface analysis. The potent inducing effect of the fungal elicitors on natamycin production was confirmed. When fungal elicitor from Aspergillus niger was added to a final concentration of 122 μg/mL after 18.3 h natamycin fermentation and incubated for 78.5 h, the observed average yield of natamycin in fermentation broth was 2.89 g/L, 1.68 times higher than the blank control.

Key words: fungal elicitor, fermentation, natamycin, response surface analysis

中图分类号:

TS 201.3

魏宝东，潘娅慧，王亚男，王利艳. 促进纳他霉素合成的真菌诱导子筛选及诱导条件优化[J]. 食品科学, 2013, 34(7): 196-200.

WEI Bao-dong,PAN Ya-hui，WANG Ya-nan，WANG Li-yan. Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions[J]. FOOD SCIENCE, 2013, 34(7): 196-200.

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促进纳他霉素合成的真菌诱导子筛选及诱导条件优化

Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions

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