食品科学 ›› 2013, Vol. 34 ›› Issue (9): 56-60.doi: 10.7506/spkx1002-6630-201309013

• 基础研究 • 上一篇    下一篇

乳清分离蛋白美拉德反应产物的体外抗氧化特性

王文琼,包怡红*,陈 颖   

  1. 东北林业大学林学院,黑龙江 哈尔滨 150040
  • 收稿日期:2012-05-27 修回日期:2013-03-17 出版日期:2013-05-15 发布日期:2013-05-07
  • 通讯作者: 包怡红 E-mail:baoyihong@163.com
  • 基金资助:

    黑龙江省博士后科研启动基金

Antioxidant Properties of Maillard Reaction Products from Whey Protein Isolate

WANG Wen-qiong,BAO Yi-hong*,CHEN Ying   

  1. College of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2012-05-27 Revised:2013-03-17 Online:2013-05-15 Published:2013-05-07
  • Contact: BAO Yi-hong E-mail:baoyihong@163.com

摘要:

以乳清分离蛋白和木糖、葡萄糖、果糖、蔗糖、麦芽糖、乳糖为美拉德反应原料,研究蛋白与糖的种类及混合比例对褐变程度的影响,并对反应产物进行抗氧化活性检测。结果表明:木糖与乳清分离蛋白按质量比2:1混合,湿热糖基化反应后,褐变程度最强,且在乳清分离蛋白与木糖按质量比2:1条件下反应后,乳清分离蛋白-木糖体系的美拉德反应产物(MRPs)具有较高还原能力和抗脂质过氧化能力及对DPPH自由基和O2-·具有较高的清除能力。

关键词: 乳清分离蛋白, 美拉德反应, 褐变, 抗氧化

Abstract:

We undertook this study to examine the effects of different sugars (xylose, glucose, fructose, sucrose, maltose
and lactose) and protein:sugar ratio on browning in the Maillard reaction of WPI (whey protein isolate). The antioxidant
activity of the MRPs was also determined. The results showed that the MRPs of WPI-xylose system at a ratio of 2:1 had
the highest browning degree, thereby showing strong reducing capacity, lipid peroxidation inhibitory effect, DPPH radical
scavenging activity, and hydroxyl free radical scavenging activity.

Key words: WPI, Maillard reaction, browning, antioxidant

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