食品科学 ›› 2013, Vol. 34 ›› Issue (6): 129-133.doi: 10.7506/spkx1002-6630-201306028

• 工艺技术 • 上一篇    下一篇

响应面分析昆仑雪菊水溶性黄酮类化合物的提取工艺

吴瑛1,2,王秀芳1,袁守亮3,*   

  1. 1.塔里木大学生命科学学院,新疆 阿拉尔 843300;2.塔里木盆地生物资源保护利用兵团省部共建国家重点实验室培育基地,新疆 阿拉尔 843300;3.塔里木大学资产管理处,新疆 阿拉尔 843300
  • 收稿日期:2011-12-08 修回日期:2013-02-01 出版日期:2013-03-25 发布日期:2013-03-01
  • 通讯作者: 袁守亮 E-mail:taru_ysl@163.com

Process Optimization by Response Surface Methodology for the Extraction of Water Soluble Flavonoids from Coreopsis tinctoria Flowers

WU Ying1,2,WANG Xiu-fang1,YUAN Shou-liang3,*   

  1. 1. College of Life Science, Tarim University, Alar 843300, China;2. Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China;3. Assets Administrative Department, Tarim University, Alar 843300, China
  • Received:2011-12-08 Revised:2013-02-01 Online:2013-03-25 Published:2013-03-01

摘要: 以新疆昆仑山产雪菊为原料,采用水作为萃取剂提取黄酮类化合物,研究提取温度料液比、提取时间和粒度大小4因素对生物黄酮含量的影响,进行响应面试验设计分析。以黄酮类化合物含量为指标,通过响应面法对提取工艺条件进行优化,最终确定水溶液萃取法提取黄酮的最优条件为提取温度90℃、料液比1:90(g/mL)、提取时间60min、粒度40目,在此条件下昆仑雪菊的水溶性黄酮含量为24.85%。

Abstract: Response surface methodology (RSM) was applied to optimize the extractions of water soluble flavonoids from Coreopsis tinctoria flowers using water as the extraction solvent. The effects of extraction temperature, material/liquid ratio, particle size and extraction time on the extraction efficiency of water soluble flavonoids were investigated. The optimal extraction conditions were extraction temperature of 90 ℃, material/liquid ratio of 1:90, extraction time of 60 min and raw material particle size of 40 mesh. The actual extraction rate of flavonoids from Coreopsis tinctoria under the optimal extraction conditions was 24.85%, which was close to the predicted value.

Key words: Coreopsis tinctoria, lavonoids, extraction, response surface methodology (RSM)

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