食品科学 ›› 2012, Vol. 33 ›› Issue (24): 122-126.doi: 10.7506/spkx1002-6630-201224025

• 工艺技术 • 上一篇    下一篇

酶法制备大豆皮微晶纤维素及其理化特性

刘恩岐,张建萍,唐仕荣,巫永华   

  1. 徐州工程学院江苏省食品资源开发与质量安全重点建设实验室,江苏 徐州 221008
  • 收稿日期:2012-06-08 修回日期:2012-11-16 出版日期:2012-12-25 发布日期:2012-12-12
  • 通讯作者: 刘恩岐 E-mail:leq@xzit.edu.cn
  • 基金资助:

    徐州工程学院科研培育项目

Enzymatic Preparation and Physicochemical Properties of Microcrystalline Cellulose from Soybean Husks

LIU En-qi,ZHANG Jian-ping,TANG Shi-rong,WU Yong-hua   

  1. Jiangsu Key Laboratory of Food Resource Development and Quality Safety, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2012-06-08 Revised:2012-11-16 Online:2012-12-25 Published:2012-12-12
  • Contact: LIU En-Qi E-mail:leq@xzit.edu.cn

摘要:

采用纤维素酶水解法制备大豆皮微晶纤维素,研究加酶量、酶解pH值、温度、时间、液料比等因素对大豆皮酶解产物中微晶纤维素(MCC)含量的影响,确定了大豆皮微晶纤维素制备的最佳工艺条件:料液比1:20(g/mL),加酶量1%,反应pH4.8,酶解温度47℃,酶解时间1h。通过理化特性和电镜扫描分析表明,制备的大豆皮MCC的平衡聚合度、持水性和表观黏度等理化特性与台湾明台商品MCC接近,且大豆皮MCC电镜扫描结构疏松,比表面积较大,从微观结构上解释了大豆皮MCC吸水后的溶胀体积较大,对肠道产生的容积作用较强,据此推断大豆皮MCC对加强肠蠕动、促进体内垃圾的排泄有更好的效果。

关键词: 大豆皮, 纤维素酶, 微晶纤维素, 理化性质

Abstract:

Microcrystalline cellulose (MCC) was prepared from soybean husks via cellulase hydrolysis. The effects of enzyme dosage, pH, time, temperature and solid-to-liquid ratio on the content of MCC in soybean husk hydrolysate were studied. The optimal conditions for MCC preparation were found to be hydrolysis at pH 4.8 and 47 ℃ for 1 h with an enzyme dosage of 1% and a solid-to-liquid ratio of 1:20 (g/mL). The prepared MCC had similar physiochemical properties such as average degree of polymerization (DP), water-holding capacity and apparent viscosity as a commercial MCC product, and showed a loose structure and a large specific surface area as shown by SEM observation. Microstructure analysis showed that the MCC obtained from soybean husks had a large swelling volume after water absorption, resulting in a good filling effect in the intestinal tract. Based on this, we speculated that MCC derived from soybean husks is more effective in enhancing peristalsis and promoting defecation.

Key words: soybean husk, cellulase, microcrystalline cellulose, physicochemical properties

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