脂质基质中植物甾醇氧化物的纯化

doi:10.7506/spkx1002-6630-201314002

食品科学 ›› 2013, Vol. 34 ›› Issue (14): 6-11.doi: 10.7506/spkx1002-6630-201314002

脂质基质中植物甾醇氧化物的纯化

姜绍通，潘影，庞敏，潘丽军，操丽丽

合肥工业大学生物与食品工程学院，合肥工业大学农产品加工研究院，安徽省农产品精深加工重点实验室，安徽合肥 230009

收稿日期:2012-06-29 修回日期:2013-05-28 出版日期:2013-07-25 发布日期:2013-08-02
通讯作者: 潘影 E-mail:jlpy2010@163.com
基金资助:
植物油中甾醇的高温氧化特性及稳定性控制研究;油料低温制油关键技术和装备研究与示范;菜籽油生物炼制关键技术研究与产业化示范;菜籽脱皮低温压榨制油技术应用及产业化

Purification of Phytosterol Oxidation Products in the Lipid Matrix

JIANG Shao-tong，PAN Ying，PANG Min，PAN Li-jun，CAO Li-li

Key Laboratory for Agriculture Processing of Anhui Province, Institute of Food Science and Engineering, School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China

Received:2012-06-29 Revised:2013-05-28 Online:2013-07-25 Published:2013-08-02
Contact: Ying PAN E-mail:jlpy2010@163.com

摘要/Abstract

摘要：

以脂质基质中植物甾醇高温氧化产物为原料，采用皂化工艺对植物甾醇氧化物进行纯化。在单因素试验基础上，以植物甾醇的质量浓度为指标，采用响应曲面法优化皂化工艺的条件，建立并分析各因素与指标的数学模型。结果表明：皂化的最佳工艺条件为KOH-乙醇溶液浓度3.22mol/L、KOH-乙醇溶液用量40.04mL/g、皂化温度69.07℃、皂化时间2.02h，在此条件下植物甾醇质量浓度达到0.1455mg/mL。

关键词: 脂质基质, 植物甾醇, 皂化, 响应曲面法, 纯化

Abstract:

Abstract：Lipid matrix was saponified for the purification of phytosterol oxidation products from it. Response surface
methodology was applied to optimize four process conditions based on phytosterol concentration. A mathematical model
was developed indicating the effect of the studied variables on phytosterol concentration. Results showed that the optimal
saponification parameters were addition of 40.04 mL/g of 3.22 mol/L KOH ethanol solution, 69.07 ℃ and 2.02 h. The final
product obtained under the optimized conditions contained 0.1455 mg/mL phytosterols.

Key words: lipid matrix, phytosterols, saponification, response surface methodology, purification

中图分类号:

TS221

姜绍通，潘影，庞敏，潘丽军，操丽丽. 脂质基质中植物甾醇氧化物的纯化[J]. 食品科学, 2013, 34(14): 6-11.

JIANG Shao-tong，PAN Ying，PANG Min，PAN Li-jun，CAO Li-li. Purification of Phytosterol Oxidation Products in the Lipid Matrix[J]. FOOD SCIENCE, 2013, 34(14): 6-11.

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脂质基质中植物甾醇氧化物的纯化

Purification of Phytosterol Oxidation Products in the Lipid Matrix

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