北五味子叶多糖的提取及抑菌活性

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 33-37.

北五味子叶多糖的提取及抑菌活性

许瑞波，高颖颖，万蓓蓓，王新新，周杰兴

淮海工学院化工学院

收稿日期:2012-06-25 修回日期:2012-09-12 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 许瑞波 E-mail:xuruibo9125@163.com
基金资助:
江苏省海洋生物技术重点建设实验室开放课题;连云港市科技攻关项目;淮海工学院博士科研启动项目

Extraction and Antibacterial Activity of Polysaccharides from Schisandra chinensis (Turcz.) Baill Leaves

Received:2012-06-25 Revised:2012-09-12 Online:2012-10-25 Published:2012-11-09

摘要/Abstract

摘要： 采用水提醇沉法，通过单因素和正交试验研究北五味子叶多糖(polysaccharides from Schisandra chinensis
(Turcz.) Baill leaves，PSL)的提取工艺，并考察PSL对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑制作用。结果表明：PSL最适宜提取工艺条件为料液比1:45(g/mL)、提取温度90℃、提取时间1h、提取2次。在此条件下，PSL的提取率为9.08%。用Sevag法对PSL进行初步提纯，用UV光谱检测脱蛋白效果，发现该法不能有效去除其所含的蛋白质，而且经Sevag法处理后的PSL色泽变黑。同时，利用红外光谱法对PSL进行表征，证明其具备一般多糖类物质及肽键的光谱特征，说明该多糖可能是以共价键与含肽键的蛋白质或多肽结合的糖复合物。抑菌实验结果表明，PSL对3种细菌具有一定的抑制活性。

关键词: 北五味子叶, 多糖, 提取工艺, 抑菌活性, 表征

Abstract: The extraction of polysaccharides from Schisandra chinensis (Turcz.) Baill leaves (PSL) was optimized using one-factor-at-a-time and orthogonal array design method. Meanwhile, the inhibitory activities of PSL against Staphylococcus aureus, Escherichia coli and Bacillus subtilis were investigated. The results showed that the optimal conditions for PSL extraction were determined as two extraction cycles with distilled water at a solid/solvent ratio of 1:45 (g/mL) at 90 ℃ for 1 h. Under these conditions, the extraction yield of PSL was 9.08%. The PSL obtained could not be effectively deproteinized by Sevag method according to UV spectroscopic analysis and its color was darkened. Meanwhile, IR spectroscopic analysis demonstrated that this PSL possessed common spectral characteristics of polysaccharides and peptide bonds, suggesting that it might be formed by conjugation of polysaccharides with proteins or polypeptides through peptide bonds. Furthermore, the polysaccharide extract possessed inhibitory activities against three bacterial strains under investigation.

Key words: Schisandra chinensis (Turcz.) Baill leaves, polysaccharides, extraction process, antibacterial activity, characterization

中图分类号:

R284.2

许瑞波，高颖颖，万蓓蓓，王新新，周杰兴 . 北五味子叶多糖的提取及抑菌活性[J]. 食品科学, 2012, 33(20): 33-37.

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