食品科学 ›› 2012, Vol. 33 ›› Issue (21): 245-248.
• 生物工程 • 上一篇 下一篇
蔡 丹，刘景圣*，郭丽丽，刘义军，陈春阳
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摘要： 利用高速逆流色谱法分离蜜环菌发酵液乙醇提取部位，筛选其最佳溶剂体系分离条件。结果表明：溶剂体系为正丁醇-乙酸乙酯-水、且体积比为1:4:5的条件下，能得到最佳分离效果，最终分离出4个峰，效果较好，4个峰的提取率分别为51.10%、1.47%、0.86%、1.80%。
关键词: 蜜环菌发酵液, 高速逆流色谱法, 溶剂体系
Abstract: This study was designed to investigate experimental conditions for high-speed countercurrent chromatography (HSCCC) separation of the ethanol extract of Armillaria mellea fermentation broth supernatant. The best separation results were achieved using a solvent system composed of butanol, ethyl acetate and deionized water (1:4:5, V/V). Four peaks were separated well with an extraction yield of 51.10%, 1.47%, 0.86% and 1.80%, respectively.
Key words: Armillaria mellea fermentation broth supernatant, high-speed countercurrent chromatography, solvent system
蔡?丹，刘景圣*，郭丽丽，刘义军，陈春阳. 高速逆流色谱法分离蜜环菌发酵上清液乙醇提取物条件的研究[J]. 食品科学, 2012, 33(21): 245-248.
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