液相色谱-串联质谱法测定食品用纸容器中全氟辛酸

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 207-209.

液相色谱-串联质谱法测定食品用纸容器中全氟辛酸

张岩1,2，庞坤2，吕品3，李挥2，卢晓宇1,*

1.北京出入境检验检疫局 2.河北省食品质量监督检验研究院 3.河北医科大学

收稿日期:2012-06-18 修回日期:2012-10-21 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 卢晓宇 E-mail:luxiaoyu@bjciq.gov.cn

Determination of Perfluorooctanoic Acid (PFOA) in Paper Food Containers by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Received:2012-06-18 Revised:2012-10-21 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 建立高效液相色谱-串联质谱结合加速溶剂萃取测定食品用纸容器中全氟辛酸的方法。样品用甲醇作为溶剂进行快速溶剂萃取。萃取液经C18固相萃取柱富集净化后，以C18柱为分离柱，以甲醇、水为流动相，梯度洗脱，电喷雾负离子模式下多反应监测模式检测。全氟辛酸在0.5～50ng/mL范围内线性良好(R2=0.9993)，回收率为90.2%～103.3%，相对标准偏差为2.74%～6.03%，检出限为0.1μg/kg(RSN=3)。该方法操作简便，灵敏度高，适用于食品用纸容器中全氟辛酸及其盐类残留分析。

关键词: 高效液相色谱-串联质谱, 全氟辛酸, 食品用纸容器, 加速溶剂萃取

Abstract: High performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with accelerated solvent extraction (ASE) was used to establish a method for quantitative detection of perfluorooctanoic acid (PFOA) in paper food containers. Samples were extracted by ASE method with methanol. The resulting extract was cleaned up using a C18 solid phase extraction (SPE) column, and separate on a chromatographic C18 column using methanol and deionized water as mobile phases by gradient elution. Instrumental analysis was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) in the multiple-reaction monitoring (MRM) mode. The developed calibration curve was linear in the range of 0.5–50 ng/mL with a correlation coefficient (r2) of 0.9993. The recovery rates for PFOA were in the range of 90.2%–103.3% with relative standard deviations of 2.74%–6.03%. The detection limit (RSN = 3) was 0.1 µg/kg. This method was easy, time-saving, sensitive and suitable for the determination the PFOA in paper foods containers.

Key words: high performance liquid chromatography tandem-mass spectrometry (HPLC-MS/MS), perfluorooctanoic acid (PFOA), food paper containers, accelerated solvent extraction (ASE)

中图分类号:

张?岩1,2，庞?坤2，吕?品3，李?挥2，卢晓宇1,*. 液相色谱-串联质谱法测定食品用纸容器中全氟辛酸[J]. 食品科学, 2012, 33(22): 207-209.

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