梯度降温对鸭梨采后果肉膜脂过氧化及褐变的影响

doi:10.7506/spkx1002-6630-201306056

食品科学 ›› 2013, Vol. 34 ›› Issue (6): 247-252.doi: 10.7506/spkx1002-6630-201306056

梯度降温对鸭梨采后果肉膜脂过氧化及褐变的影响

梁丽雅，闫师杰*，何庆峰，崔艳

天津农学院食品科学系，天津 300384

收稿日期:2012-08-07 修回日期:2013-01-21 出版日期:2013-03-25 发布日期:2013-03-01
通讯作者: 梁丽雅 E-mail:liangliya@126.com
基金资助:
国家自然科学基金面上项目

Effect of Gradient Cooling on Membrane Lipid Peroxidation and Browning of Yali Pear Flesh after Harvest

LIANG Li-ya，YAN Shi-jie*，HE Qing-feng，CUI Yan

Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China

Received:2012-08-07 Revised:2013-01-21 Online:2013-03-25 Published:2013-03-01

摘要/Abstract

摘要： 以不同成熟度鸭梨为试材，采用每2.5d降1℃，30d降至(0±1)℃的梯度降温方法，探讨梯度降温对鸭梨果肉膜脂过氧化及褐变的影响。结果表明：鸭梨采后果肉褐变指数与丙二醛(MDA)含量、自由基含量呈显著的正相关，与还原性物质含量、保护酶活性呈显著的负相关。梯度降温提高了早采果果肉超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性，一定程度上延缓了VC、还原性谷胱甘肽(GSH)含量的下降，抑制了过氧化氢(H2O2)、超氧阴离子自由基(O2－·)的积累，降低了MDA含量和膜渗透率，从而抑制了鸭梨果肉褐变。与早采鸭梨相比，晚采鸭梨果肉中VC和GSH含量高但下降快，O2－·、H2O2和MDA积累量高，SOD和POD活性较低，梯度降温促进了晚采果果肉的膜脂过氧化和褐变。所以，适时采收结合梯度降温可以很好抑制鸭梨采后果肉褐变。

关键词: 鸭梨, 果肉, 梯度降温, 褐变, 膜脂过氧化

Abstract: The effect of gradient cooling (storage at 12 ℃ and cooling them to (0 ± 1) ℃ over 30 days at a gradual interval of 1 ℃ every 2.5 days) on membrane lipid peroxidation and browning of Yali pear flesh with different maturity was explored. The results showed that flesh-browning index had significantly positive correlation with MDA and radical content, and significantly negative correlation with reducing substance and protective enzyme. Gradient cooling improved the activities of SOD, CAT and POD, delayed the decrease of vitamin C and GSH contents to a certain extent, inhibited the accumulation of H2O2, O2 －? free radicals, reduced MDA content and membrane permeability, and retarded flesh browning of Yali pears at the early stage after harvest. In contrast, the contents of vitamin C and GSH in the flesh of Yali pears at the late stage after harvest were obvious higher and revealed a fast decrease during storage. Meanwhile, higher contents of O2 －·, H2O2 and MDA, and lower activities of SOD and POD were also determined. Therefore, gradient cooling can accelerate membrane lipid peroxidation and flesh browning of Yali pears at the late stage after harvest. Timely harvesting combined with gradient cooling can efficiently inhibit the flesh browning of Yali pears during storage.

Key words: Yali pear, flesh, gradient cooling, browning, membrane lipid peroxidation

中图分类号:

梁丽雅，闫师杰*，何庆峰，崔艳. 梯度降温对鸭梨采后果肉膜脂过氧化及褐变的影响[J]. 食品科学, 2013, 34(6): 247-252.

参考文献

[1] 闫师杰,梁丽雅,陈计峦,等.降温方法对不同采收期鸭梨采后果心褐变和膜脂组分的影响[J].农业工程学报,2010, 26 (8):356-362.
[2] 梁丽雅, 闫师杰, 陈计峦, 等.鸭梨采后果实褐变的主要因素研究[J].食品与机械, 2008,24(3):48-51.
[3] CRISOSTO C H, GRANER DAVID, CRISOSTO G M. Late harvest and delayed cooling induce internal browning of ‘Ya Li’ and ‘Seuri’ Chinese pear[J]. Hortscience, 1994, 29(6): 667-670.
[4] 关军锋. 采后鸭梨衰老与膜脂过氧化作用[J]. 沈阳农业大学学报, 1994, 25(4): 418-421.
[5] 史振霞,王晶. 壳聚糖对鸭梨贮藏后期微生物数量和贮藏品质的影响[J]. 安徽农业科学,2012,40 (8):4863-4865.
[6] LI J Z，Bi D P. Effects of micro-perforated film packaging on the quality of Yali pear fruit（Pyrus bretschneideri cv. Yali）during storage[J]. Journal of Fruit Science, 2010,27(1): 57-62.
[7] 王志华, 王文辉, 佟伟, 等. 1-MCP 结合降温方法对鸭梨采后生理和果心褐变的影响[J]. 果树学报,2011,28(3): 513-517.
[8] 张爱琳,胡小松,李晓丹,等. 降温方法对鸭梨采后果肉与果心抗冷性的影响[J].食品与机械,2011, 27 (2）:114-118.
[9] 梁丽雅,胡小松,何爱红,等.降温方法对不同成熟度鸭梨果肉脂氧合酶活性和膜脂脂肪酸的影响[J].食品科技,2012,37 (4):35~39.
[10] 汤章城主编. 现代植物生理学实验指南[M]. 北京：科学出版社,1999.
[11] 曹建康,姜微波,赵玉梅. 果蔬采后生理生化实验指导[M]. 北京：中国轻工业出版社, 2007.
[12] GURI A. Ozone effect on glutathine and ascorbic acid in beans[J].Can. J. Plant Sci.,1983, 63(3):733-736
[13] 王爱国, 罗广华. 植物的超氧化物自由基与羟胺反应的定量关系[J]. 植物生理学通讯, 1990(6): 55-57.
[14] PATTERSON B D, MACKAE E A, FERGUSON I B. Estimation of hydrogen peroxide in plant extracts using titanium (Ⅳ)[J]. Annal Biochem,1984,139: 487-492
[15] BRADFORD M N. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding[J]. Annal Biochem. 1976, 72, 248-254
[16] VENISSE J S, GULLNER G, BRISSET M N. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora[J]. Plant Physiol, 2001, 125: 2164-2172.
[17] BAILLY C, BENAMAR A, CORBINEAU F, et al. Changes in malonaldehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated ageing[J]. Physiologia Plantarum, 1996, 97: 104-110.
[18] GIANNOPOLITIS C N, RIES A K. Superoxidase dismutaseI.Occurrence in higher plants. Plant Physiol, 1977, 59:309-314
[19] 赵丹莹, 申琳, 于萌萌, 等. 采后番茄果实抗氧化酶活与抗冷性的关系[J]. 食品科学, 2009, 30(14): 309-313.
[20] 周春华, 刘红霞, 韦军. 活性氧与果实成熟衰老[J]. 上海交通大学学报(农业科学版), 2002, 20(1): 77-84.
[21] 寇莉苹, 刘兴华, 赵斌, 等. 热处理对轻度加工葡萄膜脂过氧化作用的影响[J]. 农业工程学报，2006,22(4):170-173.

梯度降温对鸭梨采后果肉膜脂过氧化及褐变的影响

Effect of Gradient Cooling on Membrane Lipid Peroxidation and Browning of Yali Pear Flesh after Harvest

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