食品科学 ›› 2013, Vol. 34 ›› Issue (21): 64-68.doi: 10.7506/spkx1002-6630-201321014

• 基础研究 • 上一篇    下一篇

活性红120改性磁性壳聚糖微球对牛血清白蛋白的吸附性能

曹 淼,李忠宏*,朱 伟,齐 佳,赵静静   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 收稿日期:2012-08-18 修回日期:2013-09-17 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 李忠宏 E-mail:lizhhsteve@163.com
  • 基金资助:

    中央高校基本科研业务费专项(QN2013087);陕西省科技攻关项目(2012K02-07)

Adsorption of Bovine Serum Albumin by Reactive Red 120 Modified Magnetic Chitosan Microspheres

CAO Miao,LI Zhong-hong*,ZHU Wei,QI Jia,ZHAO Jing-jing   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Received:2012-08-18 Revised:2013-09-17 Online:2013-11-15 Published:2013-10-28
  • Contact: LI Zhong-hong E-mail:lizhhsteve@163.com

摘要:

采用反相悬浮法制备磁性壳聚糖微球,并用活性红120对其表面进行改性,以溶液pH值、离子强度、吸附时间、牛血清白蛋白(BSA)初始质量浓度为参数,研究活性红120改性磁性壳聚糖微球对牛血清白蛋白的吸附性能。结果表明:溶液pH值、离子强度、初始质量浓度、吸附时间对BSA吸附量有极显著的影响;pH5.0时BSA吸附量最大;离子强度的增大会导致BSA吸附量减小;吸附时间40min时达到吸附平衡;BSA初始质量浓度1mg/mL时达到最大平衡吸附量,为113mg/g;其吸附行为符合动力学准二级模型和Langmuir吸附等温模型;采用0.5mol/LNaCl(pH8.0)作为洗脱剂,洗脱率为99.03%;经过5次吸附-脱附循环后,活性红120改性磁性壳聚糖微球对BSA的吸附量没有显著下降。研究结果为利用磁性分离技术回收利用动物血液提供一定的理论参考。

关键词: 磁性壳聚糖微球, 牛血清白蛋白, 吸附, 活性红120

Abstract:

Magnetic chitosan microspheres were prepared by inverse suspension cross-linking method and then modified
with Reactive Red 120. Adsorption performance of the Reactive Red 120 modified magnetic chitosan microspheres were
assessed using bovine serum albumin (BSA) as the target protein. The impact of pH, ionic strength, adsorption time
and initial concentration of BSA on the amount of BSA absorbed were studied. The results showed that all pH, ionic
strength, adsorption time and initial concentration of BSA had significant impact on the amount of BSA adsorbed. The
maximum adsorption of BSA appeared at pH 5.0 and the adsorption amount decreased with increasing ionic strength. The
adsorption reached an equilibrium in 40 min, and the maximum adsorption capacity of 113 mg/g was obtained when the
initial concentration of BSA was 1 mg/mL. The adsorption behavior was fitted with pseudo second-order kinetic model
and Langmuir thermodynamic model. Using 0.5 mol/L of NaCl at pH 8.0 as the eluent, an elution efficiency of 99.03%
was achieved. The amount of BSA adsorbed by the Reactive Red 120 modified magnetic chitosan microspheres did not
decrease significantly following 5 adsorption-desorption cycles. Our results revealed a promising method for the recovery of
slaughtered animal blood by using the magnetic separation technology.

Key words: magnetic chitosan microspheres, bovine serum albumin, adsorption, reactive red 120

中图分类号: