纤维素酶辅助提取芦笋黄酮及其抗氧化活性分析

doi:10.7506/spkx1002-6630-201406004

食品科学 ›› 2014, Vol. 35 ›› Issue (6): 17-23.doi: 10.7506/spkx1002-6630-201406004

纤维素酶辅助提取芦笋黄酮及其抗氧化活性分析

董孝元1，方冬芬2，杨梅1，朱轶婷1，吴周和1,*

1.湖北工业大学生物工程学院，湖北武汉 430068；2.湖北省卫生厅卫生监督局，湖北武汉 430070

收稿日期:2012-09-13 修回日期:2014-03-10 出版日期:2014-03-25 发布日期:2014-04-04
通讯作者: 吴周和 E-mail:674089612@qq.com
基金资助:
湖北省自然科学基金项目（2010CDB02506）

Extraction and Antioxidant Activity of Asparagus Flavonoids

DONG Xiao-yuan1, FANG Dong-fen2, YANG Mei1, ZHU Yi-ting1, WU Zhou-he1,*

1. College of Bioengineering, Hubei University of Technology, Wuhan 430068, China;
2. Health Inspection and Supervision Bureau, Health Department of Hubei Province, Wuhan 430070, China

Received:2012-09-13 Revised:2014-03-10 Online:2014-03-25 Published:2014-04-04
Contact: WU Zhou-he E-mail:674089612@qq.com

摘要/Abstract

摘要：

以黄酮得率为指标，利用纤维素酶协同乙醇提取法从芦笋中提取黄酮，采用响应面设计优化最佳提取工艺参数。结果表明：当液料比50∶1（mL/g）、酶添加量0.20%、乙醇体积分数38.90%、酶解时间1.50 h、酶解温度50.0 ℃、pH 5.0时，芦笋黄酮最高得率为3.99%。当样品质量浓度达到100 μg/mL时，芦笋黄酮对羟自由基的清除率为46.08%，芦笋黄酮对超氧阴离子自由基的清除率为59.42%。小鼠体外抗氧化实验表明，20 μg/mL芦笋黄酮处理组超氧化物歧化酶（SOD）活力提高16.85%，丙二醛（MDA）含量减少2.49%。以D-半乳糖法亚急性衰老小鼠为模型，高剂量组小鼠血清和肝组织液SOD活力分别提高11.69%和27.62%，MDA含量分别减少38.04%和37.95%。

关键词: 芦笋, 黄酮, 提取, 抗氧化性

Abstract:

The present study was carried out to obtain the optimal process conditions for extracting the maximum amount
of flavonoids from asparagus. For the extraction of flavonoids, dried and powdered asparagus was refluxed with aqueous
ethanol following hydrolysis with cellulase. Various process parameters were optimized by response surface methodology.
Results indicated that the optimal extraction conditions were enzymatic hydrolysis at an enzyme dose of 0.20% for 1.5 h
at pH 5.0 followed by refluxing with 38.90% ethanol at a solvent-to-solid ratio of 50:1 (mL/g). Under these conditions,
the maximum extraction yield of flavonoids from asparagus was 3.99%. The flavonoids extracted from asparagus at the
concentration of 100 μg/mL were able to scavenge 46.08% hydroxyl radical and 59.42% superoxide anion radical. In
vitro antioxidant tests showed that treatment with concentration of 20 μg/mL flavonoids resulted in a 16.85% increase in
superoxide dismutase (SOD) activity and a 2.49% reduction in malondialdehyde (MDA) content of mouse serum. SOD
activity in serum and liver tissue homogenate supernatant from D-galactose induced mouse aging model was increased by
11.69% and 27.62% as a result of this treatment, and MDA content was reduced by 38.04% and 37.95%, respectively.

Key words: asparagus, flavonoid, extraction, antioxidant activity

中图分类号:

TS201.2

董孝元1，方冬芬2，杨梅1，朱轶婷1，吴周和1,*. 纤维素酶辅助提取芦笋黄酮及其抗氧化活性分析[J]. 食品科学, 2014, 35(6): 17-23.

DONG Xiao-yuan1, FANG Dong-fen2, YANG Mei1, ZHU Yi-ting1, WU Zhou-he1,*. Extraction and Antioxidant Activity of Asparagus Flavonoids[J]. FOOD SCIENCE, 2014, 35(6): 17-23.

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纤维素酶辅助提取芦笋黄酮及其抗氧化活性分析

Extraction and Antioxidant Activity of Asparagus Flavonoids

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