食品科学 ›› 2013, Vol. 34 ›› Issue (3): 271-275.

• 营养卫生 • 上一篇    下一篇

豆渣膳食纤维及豆渣超微化制品对小鼠肠道菌群的影响

吴占威,胡志和   

  1. 天津商业大学生物技术与食品科学学院
  • 收稿日期:2012-09-21 修回日期:2013-01-12 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 胡志和 E-mail:hzhihe@tjcu.edu.cn

Effects of dietary fiber from okara and superfine okara on intestinal microflora of mice

  • Received:2012-09-21 Revised:2013-01-12 Online:2013-02-15 Published:2017-12-29

摘要: 用豆渣膳食纤维及豆渣超微化制品灌胃BALB/c小鼠,研究其对小鼠肠道菌群的影响。正常对照组用生理盐水进行灌胃,试验组分别以低剂量(0.5g/kg)、高剂量(2.5g/kg)灌胃水溶性膳食纤维(SDF)、非水溶性膳食纤维(IDF)、超微粉碎豆渣(SPO)和螺杆挤压-超微粉碎豆渣(ESPO)。试验期间,每周同一时间取小鼠粪便,采用选择性培养基检测小鼠粪便中乳酸杆菌、双歧杆菌、肠杆菌和肠球菌的数量。结果表明,灌胃的各个阶段,各试验组在灌胃豆渣膳食纤维及豆渣制品后对小鼠的肠道菌群均有较大影响,其中高剂量IDF、低剂量SDF和低剂量的ESPO对小鼠肠道菌群调理作用较为明显,在增加乳酸杆菌和双歧杆菌的同时,一定程度上抑制了肠球菌和肠杆菌的增长。

Abstract: In order to study the effects of dietary fiber from okara and superfine okara on intestinal microflora of mice, BALB/c mice were divided into groups for gavage. The control group were given gavages with normal saline, the experimental groups were given gavages with Soluble Dietary Fiber(SDF)、Insoluble Dietary Fiber(IDF)、Superfine Pulverization Okara(SPO) and Extrusion-Superfine Pulverization Okara(ESPO) respectively at low dose(0.5g/kg) and high dose(2.5g/kg). During the experiment, the selective medium was used to detect the quantity of lactobacillus、Bifidobacterium、Enterobacter and Enterococcus in the fecal of mice at the same time every week. The results showed that each treatment group was susceptible to intestinal microflora after dietary fiber from okara and superfine okara being given perfusion in every stage. The opsonization of high-dose IDF group、low-dose SDF group and low-dose ESPO group were better, lactobacillus and Bifidobacterium were added, however, Enterobacter and Enterococcus were suppressed partly.

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