响应面法优化双歧杆菌B04代谢产细菌素的发酵条件

食品科学 ›› 2013, Vol. 34 ›› Issue (3): 147-152.

响应面法优化双歧杆菌B04代谢产细菌素的发酵条件

刘国荣¹,张郡莹¹,王成涛²,李平兰³,任丽¹

1. 北京工商大学
2. 北京工商大学食品学院
3. 中国农业大学

收稿日期:2012-09-14 修回日期:2013-01-07 出版日期:2013-02-15 发布日期:2017-12-29
通讯作者: 李平兰 E-mail:lipinglan420@126.com
基金资助:
国家自然科学基金项目;国家“863”计划项目

Optimization of Fermentation Conditions of Bifidobacterium animal B04 for Bacteriocin Production by Response Surface Methodology

Received:2012-09-14 Revised:2013-01-07 Online:2013-02-15 Published:2017-12-29

摘要/Abstract

摘要： 本研究以分离自中国广西巴马长寿老人肠道的产细菌素动物双歧杆菌（Bifidobacterium animal）B04为研究对象，以单核细胞增生李斯特菌为细菌素抑菌活性测试指示菌，以相对抑菌效价为考察指标，对菌株B04所产细菌素的发酵条件进行了优化。首先考察了不同接种量、培养时间、培养温度及起始pH值等单因素对细菌素合成的影响，确定培养时间、培养温度及起始pH值三个因素对细菌素的产量影响较为显著，选取这三因素继续进行响应面试验优化。优化后的最佳培养条件确定为：接种量为1%、培养温度36℃、发酵29h、起始pH值6.3。在此优化条件下，细菌素效价高达3479.25AU/mL，比优化前（1032.66 AU/mL）提高了2.37倍。同时，最优发酵条件下获得的实验结果与模型预测值相吻合，说明所建立的回归模型是切实可行的。

Abstract: The fermentation condition of Bifidobacterium animal B04 isolated from centenarians’ intestine, were optimized for bacteriocin production. The bacteriocin inhibitory activity (AU/mL) was used as evaluation index and quantified against L. monocytogenes ATCC 35152. Firstly, the effects of the single factors (inoculum, initial pH, fermentation temperature and time) on bacteriocin production from strain B04 were examined. Based on these single factor tests, response surface methology with three variables of initial pH, fermentation temperature and time was employed to optimize the fermentation condition for bacteriocin production. Results showed the optimal conditions were inoculum 1%, initial pH 6.3, fermentation 29h at 36℃. Under these optimal conditions, the bacteriocin inhibitory activity was increased by 2.37 times and reached up to 3479.25AU/mL. Finally, the consistent results between the prediction and experiments indicated the established model in this study is feasible.

中图分类号:

刘国荣张郡莹王成涛李平兰任丽. 响应面法优化双歧杆菌B04代谢产细菌素的发酵条件[J]. 食品科学, 2013, 34(3): 147-152.

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