硝酸银诱导青仁乌生成大豆抗毒素的机理

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 18-22.

硝酸银诱导青仁乌生成大豆抗毒素的机理

冯东岳1,2，石波1,*

1.中国农业科学院饲料研究所 2.全国水产技术推广总站

收稿日期:2012-09-29 修回日期:2012-11-13 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 石波 E-mail:shibo@mail.caas.net.cn
基金资助:
国家自然科学基金面上项目

Mechanism of Silver Nitrate Induced Glyceollin Production in Black Soybeans during Germination

Received:2012-09-29 Revised:2012-11-13 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 目的：以大豆异黄酮(大豆苷和大豆苷元)为指标，研究硝酸银诱青仁乌生成大豆抗毒素的机理。方法：以0.01mol/L硝酸银为诱导剂，采用分析型高效液相法测定青仁乌大豆抗毒素的生成量，同时检测大豆苷和大豆苷元的含量变化，以及分析丙二酰大豆苷和丙二酰染料木苷的变化规律，进而初步分析青仁乌大豆抗毒素的生成机理。结果：大豆苷元是生成大豆抗毒素的重要前体物质，大豆苷、丙二酰大豆苷和丙二酰染料木苷对大豆抗毒素的生成具有一定影响。结论：检测大豆中大豆苷元的含量，可为选择大豆品种诱导生成大豆抗毒素提供依据。

关键词: 高效液相色谱法, 大豆抗毒素, 大豆苷, 大豆苷元

Abstract: The objective of this study was to clarify the mechanism of silver nitrate-induced glyceollin synthesis in black soybeans during germination. Black soybeans were treated with 0.01 mol/L silver nitrate to induce the production of glyceollin during cultivation. The contents of glyceollin, daidzin, daidzein, malonylgenstin, and malonyldaidzein in cultivated black soybeans were determined by HPLC. Daidzein was an important precursor for the synthesis of glyceollin. Daidzin, malonylgenstin and malonyldaidzein had an important influence on the synthesis of glyceollin. In conclusion, daidzein content can provide evidence for the selection of black soybean cultivars suitable for silver nitrate induced glyceollin production.

Key words: HPLC, glyceollins, daidzin, daidzein

中图分类号:

Q819

冯东岳1,2，石?波1,*. 硝酸银诱导青仁乌生成大豆抗毒素的机理[J]. 食品科学, 2012, 33(23): 18-22.

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