食品科学 ›› 2012, Vol. 33 ›› Issue (23): 51-54.

• 基础研究 • 上一篇    下一篇

猕猴桃籽油体外抗氧化活性

李加兴1,余 娇1,黄 诚1,周炎辉2,肖文军3   

  1. 1.吉首大学食品科学研究所 2.湖南奇异生物科技有限公司 3.湖南农业大学园艺园林学院
  • 收稿日期:2012-10-11 修回日期:2012-11-12 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 李加兴 E-mail:jslijiaxing@sohu.com
  • 基金资助:

    “十二五”国家农业科技支撑计划;湖南省高校科技创新团队支持计划

in vitro Antioxidant Activity of Kiwifruit Seed Oil

  • Received:2012-10-11 Revised:2012-11-12 Online:2012-12-15 Published:2012-12-12
  • Contact: Jia-Xing LI E-mail:jslijiaxing@sohu.com

摘要: 以猕猴桃籽油为原料,采用体外抗氧化实验模型研究其抗氧化活性。通过测定猕猴桃籽油还原力和DPPH自由基、超氧阴离子自由基(O2-·)、H2O2以及羟自由基(·OH)清除实验,以VC为阳性对照,探讨猕猴桃籽油的抗氧化性能。结果表明:猕猴桃籽油的还原能力随其质量浓度的增加而增强,对DPPH自由基、O2-·、H2O2以及·OH的清除率随质量浓度的增加而升高,其中对O2-·的清除效果比VC更明显,对其他自由基的清除效果却比VC弱;猕猴桃籽油对O2-·、H2O2、·OH的IC50分别为0.078、0.1、0.2mg/mL,表明猕猴桃籽油清除O2-·的效果最好。

关键词: 猕猴桃籽油, 抗氧化活性, 还原力, 自由基

Abstract: The in vitro antioxidant activity of kiwifruit seed oil was evaluated by reducing power assay and DPPH radical, superoxide anion radical, hydrogen peroxide and hydroxyl radical scavenging assays and compared with vitamin C as a positive control. The results showed that the reducing power and scavenging activity against DPPH radicals, superoxide anion radicals, hydrogen peroxide and hydroxyl radicals of kiwifruit seed oil were directly proportional to concentration. Kiwifruit seed oil could scavenge superoxide anion radicals better than vitamin C but was less effective in scavenging DPPH radicals, hydrogen peroxide and hydroxyl radicals. The IC50 values against superoxide anion radicals, hydrogen peroxide and hydroxyl radicals were 0.078, 0.1 mg/mL and 0.2 mg/mL, respectively. Therefore, kiwifruit seed oil had the strongest scavenging effect against superoxide anion radicals.

Key words: kiwifruit seed oil, antioxidant activity, reducing power, free radicals

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