食品科学 ›› 2012, Vol. 33 ›› Issue (23): 70-74.

• 基础研究 • 上一篇    下一篇

大枣中四种功能性糖分的分离纯化及其单糖组成分析

赵子青,原 超,林勤保*   

  1. 山西大学应用化学研究所
  • 收稿日期:2012-07-09 修回日期:2012-11-12 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 林勤保 E-mail:qblin@sxu.edu.cn
  • 基金资助:

    林业公益性行业科研专项

Isolation, Purification, and Structural Analysis of Four Functional Saccharides from Chinese Dates

  • Received:2012-07-09 Revised:2012-11-12 Online:2012-12-15 Published:2012-12-12

摘要: 大枣粗低聚糖用强碱性阴离子交换树脂脱色,再经DEAE-52纤维素柱初步纯化,得到3个组分JuBO1、JuBO2、JuBO3,其中JuBO1过Sephadex G-25凝胶柱层析进一步纯化,得JuBO1-1、JuBO1-2两个单一组分,同时JuBO2、JuBO3过Sephadex G-15凝胶柱层析脱盐。4种大枣功能性糖分经冷冻干燥后用气相色谱-质谱联用法、高效液相色谱法测定,得到其单糖组成,其中JuBO1-1的单糖组成为阿拉伯糖、甘露糖、半乳糖和葡萄糖,JuBO1-2单糖组成为阿拉伯糖、鼠李糖、果糖、甘露糖、半乳糖和葡萄糖,JuBO2的单糖组成为阿拉伯糖、果糖和葡萄糖,JuBO3的单糖组成为阿拉伯糖、鼠李糖、果糖和葡萄糖。将分离纯化得到的4种样品分别进行凝胶色谱分析,结果得到JuBO1-1的平均分子质量为5952D,JuBO1-2的平均分子质量为545D,JuBO2的平均分子质量为108629D,JuBO3的平均分子质量为2624D。

关键词: 大枣, 低聚糖, 多糖, 分离, 纯化, 结构分析

Abstract: Crude oligosaccharides were obtained from Chinese dates by hot water extraction, decolorized using (201 × 7) 717 type strongly alkaline anion exchange resin, and purified on DEAE-52 cellulose column to obtain three fractions, JuBO1, JuBO2 and JuBO3. JuBO1 was further purified by Sephadex G-25 column chromatography to obtain two single components, JuBO1-1 and JuBO1-2. Besides, JuBO2 and JuBO3 were desalted by Sephadex G-15 column chromatography, along with JuBO1-1 and JuBO1-2, lyophilized and analyzed by HPLC and GC-MS for monosaccharide composition. The monosaccharide composition consisted of arabinose, mannose, galactose and glucose for JuBO1-1, arabinose, rhamnose, fructose, mannose, galactose and glucose for JuBO1-2, arabinose, fructose and glucose for JuBO2 and arabinose, rhamnose, fructose and glucose for JuBO3. The average molecular weights of JuBO1-1, JuBO1-2, JuBO2 and JuBO3 were determined by gel filtration chromatography to be 5952, 545, 108629 D and 2624 D, respectively.

Key words: Chinese date, oligosaccharide, polysaccharide, separation, purification, structural analysis

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