食品科学 ›› 2012, Vol. 33 ›› Issue (23): 287-290.

• 营养卫生 • 上一篇    下一篇

口服透明质酸对佐剂性关节炎的作用

陈 洁1,增田泰伸2,臼田美香2,木村守2,徐文斌1,郑潮鹏1,洪怡蓝1,任晓静1,奚印慈1,*   

  1. 1.上海海洋大学食品学院 2.日本丘比株式会社
  • 收稿日期:2012-07-10 修回日期:2012-11-12 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 奚印慈 E-mail:xiyinci@gmail.com

Therapeutic Effect of Oral Administration of Hyaluronic Acid on Adjuvant Arthritis in Mice

  • Received:2012-07-10 Revised:2012-11-12 Online:2012-12-15 Published:2012-12-12

摘要: 目的:探讨口服透明质酸(HA)对小鼠佐剂性关节炎(AA)的治疗作用。方法:将40只6周龄的雄性小鼠随机分成4个小组,正常组、模型组、HA组和双氯芬酸钠组。通过给模型组、HA组和双氯芬酸钠组小鼠右后足注射弗氏完全佐剂(FCA)建立佐剂性关节炎模型,经连续4周口服透明质酸进行治疗后,分别对小鼠的体质量、足跖厚度进行测量,观察小鼠膝关节肿胀程度并评分,同时采用酶联免疫吸附实验(ELISA)检测小鼠血清中白介素-1(IL-1)和HA的含量。结果:口服HA能显著降低小鼠足跖厚度和关节炎指数,同时血清中IL-1和HA含量亦显著降低;并且各项指标与双氯芬酸钠无显著性差异。结论:口服透明质酸能有效地缓解小鼠膝关节的肿胀,降低血清中IL-1的含量,增强活动能力,因而对小鼠佐剂性关节炎具有良好的治疗作用。

关键词: 口服透明质酸, 动物模型, 佐剂性关节炎

Abstract: Objective: To examine the therapeutic effect of oral administration of hyaluronic acid (HA) on adjuvant arthritis (AA) in mice. Methods: Forty 6-weeks-old healthy mice were randomly divided into 4 groups: normal, model, HA and diclofenac groups. AA mouse models were created by injection of Fluoride’s complete adjuvant (FCA) into the right hind paw. After 4 successive weeks of oral HA administration, mouse body weight and footpad thickness were measured and knee swelling was observed and scored. In addition, serum levels of interleukin-1 (IL-1) and HA were assayed. Results: Oral HA administration resulted in a significant reduction in mouse footpad thickness and arthritis index and serum levels of IL-1 and HA and showed no significant differences in various indicators compared with diclofenac. Conclusions: Oral HA administration is effective at alleviating knee swelling, lowering serum interleukin-1 level and enhancing physical activities in AA mice and thus has a powerful therapeutic effect on adjuvant arthritis.

Key words: oral administration of hyaluronic acid, animal model, adjuvant arthritis

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