食品科学 ›› 2012, Vol. 33 ›› Issue (23): 291-296.

• 营养卫生 • 上一篇    下一篇

香菇超细粉对人体细胞因子的调节作用

孙 勇1,2,王连芬2,徐增慧2,马永征2,杨文建3,马 宁3,赵立艳1,安辛欣1,方 勇3,庞广昌4,耿超宇4,胡秋辉1,3,*   

  1. 1.南京农业大学食品科技学院 2.北京市食品研究所 3.南京财经大学食品科学与工程学院 4.天津商业大学生物技术与食品科学学院  
  • 收稿日期:2012-11-24 修回日期:2012-11-28 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 孙勇 E-mail:sy183509@163.com
  • 基金资助:

    国家现代农业产业技术体系建设专项

Regulatory Effect of Lentinus edodes Ultrafine Powder on Human Cytokines

  • Received:2012-11-24 Revised:2012-11-28 Online:2012-12-15 Published:2012-12-12

摘要: 通过青年志愿者服用香菇超细粉,使用Millipore Human Kit和液体芯片扫描仪检测服用前后血清中39种细胞因子浓度的变化,研究香菇对人体细胞因子的调节作用。结果表明:食用5g香菇后EGF浓度显著升高、IP-10浓度显著降低,食用10g香菇后G-CSF、IL-12(p70)、IP-10、MCP-1 4种细胞因子浓度显著降低,食用15g香菇后Eotaxin、G-CSF、IL-12(p70)、MCP-1、MIP-1β、TGF-α 6种细胞因子浓度显著降低。根据显著变化的细胞因子进行信息检索,按照其合成与分泌细胞及其作用的靶细胞数据,构建食用3种剂量香菇后人体细胞通讯网络图。从细胞通讯网络图可以看出,食用5g香菇具有对人体免疫的双向调节作用,食用10g和15g香菇均具有下调免疫的作用;不同剂量组显著变化的细胞因子不同,细胞通讯规律也不同,高剂量香菇对免疫系统的调节作用较强;推断香菇超细粉具有抑制炎症发生、抗过敏、抗动脉粥样硬化、抗肿瘤的功能。

关键词: 香菇, 细胞因子, 细胞通讯网络, 调节作用

Abstract: The immunoregulatory effect of Lentinus edodes ultrafine powder in 12 healthy subjects was explored by analyzing changes in serum levels of 39 cytokines using a Millipore Human Kit and a liquid chip scanner. Lentinus edodes ingestion at a dose of 5 g resulted in a significant increase in EGF level and a significant reduction in IP-10 level, G-CSF, IL-12(p70), IP-10 and MCP-1 levels showed a significant decrease after ingestion of 10 g of Lentinus edodes, and significant decreases in Eotaxin, G-CSF, IL-12(p70), MCP-1, MIP-1β and TGF-α were observed when the dose was further increased to 15 g. Through online information searching, we obtained data on the specific target cells of cytokines found to show significant changes in response to Lentinus edodes ingestion at each dose and those responsible for their synthesis and secretion. Based on the obtained data, we constructed cell-cell communication networks that represent intercellular interactions. The constructed intercellular communication networks demonstrated that ingestion of 5 g of Lentinus edodes had dual immunoregulatory effects in humans, while a down-regulatory effect on human immune functions was found at doses of 10 g and 15 g. Moreover, different cytokines significantly changed and there were different cell-cell communication regulations in response to different doses of Lentinus edodes. In conclusion, high-dose Lentinus edodes ingestion has powerful regulatory effects on human immune system. Furthermore, it is speculated that Lentinus edodes has anti-inflammatory, anti-agllergic, anti-atherosclerotic and anti-tumor functions.

Key words: Lentinus edodes, cytokines, cell-cell communication network, regulatory effect

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