Abstract: The bamboo resources are abundant in China, and bamboo juice has high edible and medicinal value, therefore, the research and development of bamboo juice products has important practical significance. In this study，the fresh bamboo juice was adopted by the method of extraction of living water from Yunnan Banna dendrocalamus, and then made into freeze-dried powder by freeze-drying, the nutritional composition was investigated next. The results showed that the bamboo juice contained, Protein (10.08%)，Flavonoids (77.36mg/100g), polyphenols (38.70mg/100g), polysaccharides (46.95mg/100g), Si (0.25%)，Ca (1.27%)、Mg (0.70%)，Ge (0.65mg/kg) and other mineral elements, and also the 14 kinds of amino acids (6.48%). The finding provided a scientific basis for the research and development of bamboo juice cultivated in Yunnan Banna.