食品科学 ›› 2013, Vol. 34 ›› Issue (7): 275-278.doi: 10.7506/spkx1002-6630-201307058

• 营养卫生 • 上一篇    下一篇

不同分子质量小麦胚芽多肽的体内抗氧化活性

王才立,张志国,王成忠*,杨爱华   

  1. 山东轻工业学院食品与生物工程学院,山东 济南 250353
  • 收稿日期:2012-09-26 修回日期:2013-03-13 出版日期:2013-04-15 发布日期:2013-03-20
  • 通讯作者: 王成忠 E-mail:caili0628@163.com
  • 基金资助:
    山东省自然科学基金项目(ZR2011CM039);山东省国际科技合作计划项目

Antioxidant Activity of Polypeptides with Different Molecular Weights from Wheat Germ

WANG Cai-li,ZHANG Zhi-guo,WANG Cheng-zhong*,YANG Ai-hua   

  1. College of Food and Bio-engineering, Shandong Polytechnic University, Jinan 250353, China
  • Received:2012-09-26 Revised:2013-03-13 Online:2013-04-15 Published:2013-03-20
  • Contact: WANG Cheng-zhong E-mail:caili0628@163.com

摘要: 研究不同分子质量小麦胚芽多肽的体内抗氧化活性。利用超滤和纳滤技术对通过复合酶法制备的小麦胚芽多肽进行分级分段和纯化。通过建立小白鼠的氧化损伤模型,将170只小鼠随机分成17组(正常对照组、模型对照组、不同分子质量以及不同剂量的小麦胚芽多肽实验组),除正常对照组外,其余各组进行腹部注射600mg/(kg·d)D-半乳糖建立氧化损伤模型,5种不同分子质量的多肽组灌胃低、中、高剂量的小麦胚芽多肽,实验周期40d,眼球取血处死小鼠,测定血清、肝脏中的超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量和总抗氧化能力(T-AOC),研究不同分子质量小麦胚芽多肽的体内抗氧化活性。结果表明:小麦胚芽多肽能够使小鼠血清和肝脏中SOD、GSH-Px以及T-AOC的指标升高,降低小鼠血清和肝脏中脂质过氧化物的MDA含量,具有良好的抗氧化活性。总体来说,随着多肽质量浓度的升高,抗氧化活性也随之增强,分子质量在2000D以下的小麦胚芽多肽具有较强的抗氧化活性。

关键词: 小麦胚芽多肽, 抗氧化性, 超滤, 纳滤, 分段, 氧化损伤模型

Abstract: In this study, the antioxidant activity of polypeptides with different molecular weights from wheat germ was investigated. Ultrafiltration and nanofiltration were used to sub-classification and purification of wheat germ peptides prepared by composite enzymatic hydrolysis. Meanwhile, oxidative damage mouse models were established and 170 mice were randomly divided into 17 groups (normal control group, model control group, wheat germ polypeptide groups with different molecular weights and different concentrations. Except the normal control group, all the mice were injected with D-galactoseat a dosage of 600 mg/(kg?d) to establish oxidative damage model. The mice in polypeptides groups were administered with polypeptides with five molecular weights at low, medium and high doses. Forty days later, the mice were sacrificed and exsanguinated from eyeballs, and superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA), and total antioxidant capacity (T-AOC) in serum and liver were determined to explore antioxidant activity of wheat germ peptides. The results showed that wheat germ peptides could improve the activities of SOD and GSH-Px as well as T-AOC in serum and liver, and reduce MDA level in serum and liver, suggesting good antioxidant activity. In addition, increasing the peptide concentration could also improve the antioxidant activity. Moreover, wheat germ peptides with molecular weight of 2000 D revealed better antioxidant activity.

Key words: wheat germ polypeptide, antioxidant activity, ultrafiltration, nanofiltration, segments, oxidative damage model

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