食品科学 ›› 2013, Vol. 34 ›› Issue (21): 259-263.doi: 10.7506/spkx1002-6630-201321052

• 生物工程 • 上一篇    下一篇

梅利斯丛梗孢酵母发酵生产赤藓糖醇的条件优化

蔡 威1,张建志1,江正强2,闫巧娟1,*   

  1. 1.中国农业大学工学院,北京 100083;2.中国农业大学食品科学与营养工程学院,北京 100083
  • 收稿日期:2012-09-08 修回日期:2013-09-23 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 闫巧娟 E-mail:jiangyinuo@cau.edu.cn
  • 基金资助:

    教育部新世纪人才支持计划

Optimization of Fermentation Conditions for Erythritol Production by Moniliella mellis and Wickerhamomyces anomalus

CAI Wei1,ZHANG Jian-zhi1,JIANG Zheng-qiang2,YAN Qiao-juan1,*   

  1. 1. College of Engineering, China Agricultural University, Beijing 100083, China;
    2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2012-09-08 Revised:2013-09-23 Online:2013-11-15 Published:2013-10-28
  • Contact: YAN Qiao-juan E-mail:jiangyinuo@cau.edu.cn

摘要:

研究梅利斯丛梗孢酵母(Moniliella mellis)耦合异常威克汉姆酵母(Wickerhamomyces anomalus)发酵生产赤藓糖醇。通过单因素试验优化梅利斯丛梗孢酵母C-703产赤藓糖醇的发酵条件,得到最佳摇瓶发酵条件:培养基为葡萄糖250g/L和酵母提取物17.5g/L,初始pH5.5,250mL摇瓶装液20mL,温度28℃。在该条件下,发酵6d赤藓糖醇产量为84.6g/L。梅利斯丛梗孢酵母在5L发酵罐补料发酵10d,赤藓糖醇产量达114g/L。进一步将发酵液接入异常威克汉姆酵母C-430进行二次发酵,除去绝大部分副产物丙三醇,再经精制和结晶得到赤藓糖醇晶体,转化率为93.2%。结果表明双酵母耦合发酵生产赤藓糖醇具有较大的应用前景。

关键词: 梅利斯丛梗孢酵母, 异常威克汉姆酵母, 赤藓糖醇, 发酵

Abstract:

Erythritol production by sequential fermentation with Moniliella mellis and Wickerhamomyces anomalus was
investigated in this report. The optimal fermentation conditions for Moniliella mellis C-703 were obtained by one-factor-ata-
time design as follows: the fermentation medium containing glucose 250 g/L and yeast extract 17.5 g/L at initial pH 5.5, 20 mL
of the medium in a 250-mL flask and 28 ℃. After 6 d of fermentation under these conditions, the yield of erythritol was 84.6 g/L.
This process was followed by fed-batch fermentation with the same strain in a 5-L fermentor for 10 d, yielding 114 g/L of
erythritol fermented broth. The second fermentation was performed by inoculation of Wickerhamomyces anomalus C-430.
As a result, most of the byproduct glycerol was removed and erythritol crystals were obtained with a yield of 93.2% after
refining and crystallization. Hence sequential fermentation with Moniliella mellis and Wickerhamomyces anomalus has a
promising prospect for the application in erythritol production.

Key words: Moniliella mellis, Wickerhamomyces anomalus, erythritol, fermentation

中图分类号: