食品科学 ›› 2013, Vol. 34 ›› Issue (9): 189-193.doi: 10.7506/spkx1002-6630-201309039

• 生物工程 • 上一篇    下一篇

榛子仁蛋白酶解工艺的优化及酶解物抗氧化能力的研究

郭庆启1,2,张 娜2,姜元松1,邹传山1   

  1. 1.东北林业大学林学院,黑龙江 哈尔滨 150040;
    2.哈尔滨商业大学 食品科学与工程黑龙江省高校重点实验室,黑龙江 哈尔滨 150076
  • 收稿日期:2012-10-07 修回日期:2013-03-18 出版日期:2013-05-15 发布日期:2013-05-07
  • 通讯作者: 郭庆启 E-mail:qingqiguo@126.com
  • 基金资助:

    中央高校基本科研业务费专项(DL12BA04);黑龙江省高校食品科学与工程重点实验室开放课题

Optimization of Enzymatic Hydrolysis of Hazelnut Kernel Protein and Antioxidant Capacity of Its Hydrolysate

GUO Qing-qi1,2,ZHANG Na2,JIANG Yuan-song1,ZOU Chuan-shan1   

  1. 1. School of Forestry, Northeast Forestry University, Harbin 150040, China;
    2. Key Laboratory of Food Science and Engineering of Heilongjiang Province, Harbin University of Commerce, Harbin 150076, China
  • Received:2012-10-07 Revised:2013-03-18 Online:2013-05-15 Published:2013-05-07
  • Contact: Qing-Qi GUO E-mail:qingqiguo@126.com

摘要:

以东北毛榛子为原料,通过响应面法对Alcalase碱性蛋白酶水解榛子仁蛋白的条件进行优化并建立回归模型,得到最佳水解条件为:酶与底物比3720U/g、酶解温度50.6℃、酶解pH 8.4,此工艺条件下的水解度预测值为14.41%,水解度实测值为(14.38±0.06)%。抗氧化能力测定结果表明:榛子仁蛋白酶解物的总还原能力约为VC的70%,清除DPPH自由基的半数抑制质量浓度(IC50)值为0.7311mg/mL,是VC值的138倍。

关键词: 榛子仁蛋白, Alcalase碱性蛋白酶, 水解, 响应面, 抗氧化

Abstract:

The hydrolysis of defatted kernels of Corylus mandshurica grown in Northeast China with alcalase was
optimized by response surface methodology. A regression model was created. The optimal hydrolysis conditions were found
to be 3720 U/g, 50.6 ℃ and 8.4 for enzyme/substrate ratio, temperature and pH, respectively. The maximum predicted degree of
hydrolysis under the optimal conditions was 14.41%, compared to the actual value of (14.38 ± 0.06)%. The total reducing power
of the hydrolysate obtained was 70% of that of VC. In DPPH radical scavenging assays, its IC50 was 0.7311 mg/mL, about 138
times higher than that observed for VC.

Key words: hazelnut kernel protein, acalase, hydrolysis, response surface analytical methodology, antioxidation

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