食品科学 ›› 2013, Vol. 34 ›› Issue (21): 121-124.doi: 10.7506/spkx1002-6630-201321025

• 基础研究 • 上一篇    下一篇

乳酸亚铁对两种致病菌的生长抑制及细胞膜通透性的影响

杨 静,于 新*,杨鹏斌,刘淑宇,黄晓敏   

  1. 仲恺农业工程学院轻工食品学院,广东 广州 510225
  • 收稿日期:2012-10-08 修回日期:2013-10-11 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 于新 E-mail:yuxin1959@yahoo.com.cn

Growth Inhibition and Cell Membrane Damage of Two Foodborne Pathogens by Ferrous Lactate

YANG Jing,YU Xin*,YANG Peng-bin,LIU Shu-yu,HUANG Xiao-min   

  1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
  • Received:2012-10-08 Revised:2013-10-11 Online:2013-11-15 Published:2013-10-28
  • Contact: YU Xin E-mail:yuxin1959@yahoo.com.cn

摘要:

以金黄色葡萄球菌和铜绿假单胞杆菌为供试菌,通过测定其生长曲线和胞内物质外泄情况,研究乳酸亚铁溶液对这两种具有代表性的食品致病菌的生长抑制及细胞膜通透性的影响。结果表明:经过乳酸亚铁溶液处理后,菌体的生长受到抑制,并未达到正常高峰;与对照组相比,乳酸亚铁溶液处理组细菌培养液的电导率增加>14%,菌液的OD260nm增加>42%,培养液中还原糖含量增加>40%,可溶性蛋白质含量增加>6.87%。由此表明,乳酸亚铁溶液能够增大菌体细胞膜的通透性,导致胞内物质大量泄漏,从而抑制菌体的生长。

关键词: 乳酸亚铁, 金黄色葡萄球菌, 铜绿假单胞杆菌, 生长抑制, 细胞膜通透性

Abstract:

The effect of ferrous lactate on growth inhibition and cell membrane damage of Staphylococcus aureus (G+)
and Pseudomonas aeruginosa (G-) were investigated through the determination of growth curve and leakage of intracellular
substances. As the results showed, the growth of the pathogenic bacteria was inhibited after ferrous lactate treatment,
accompanied by increases in the conductivity, OD260nm, reducing sugar content and soluble sugar content by 14%, 42%, 40%
and 6.87%, respectively, as compared to the control group. These observations indicated that ferrous lactate solution could
damage cell membrane, thus resulting in an increase in the permeability of cell membrane and the release of large amounts
of intracellular contents, and finally inhibiting bacterial growth.

Key words: ferrous lactate, Staphylococcus aureus, Pseudomonas aeruginosa, growth inhibition, cell membrane damage

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