Abstract: Microwave-assisted enzyme conditions of the preparation of high F value corn peptide were optimized. The raw material corn gluten meal was degreased by supercritical CO2 fluid,and Alcalase 2.4L and papain were used to zymolysis. When the concentration of substrate was 9%, Alcalase 2.4L enzyme amount was 3%(E/S), pH was 9.0 ,microwave power was 300 W, microwave time was 2.5 min, corn peptide yield was 23.8g/100 gCGM .When papain amount was 4%(E/S), pH was 7.5, microwave power was 100 W,microwave time was 3.0 min, the alkali expenditure were 6.22 mL/100 mL. The F value of the corn peptides made by microwave-assisted enzyme were 27.15,increased 21% compared with the traditional water bath enzyme, and shorten the enzymatic hydrolysis time.