食品科学 ›› 2013, Vol. 34 ›› Issue (3): 141-146.

• 基础研究 • 上一篇    下一篇

金花茶多糖单一成分的化学结构特征解析

林华娟1,田晓春2,秦小明2,路垚1   

  1. 1. 广东海洋大学食品科技学院
    2. 广东海洋大学
  • 收稿日期:2012-08-20 修回日期:2013-01-12 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 林华娟 E-mail:huajuanlin@vip.163.com
  • 基金资助:
    淀粉与植物蛋白深加工教育部工程研究中心开放基金资助项目;防城港市科学研究与技术开发计划

Chemical structure character of a purified polysaccharide from Leaves of Camellia chrysantha (Hu) Tuyama

  • Received:2012-08-20 Revised:2013-01-12 Online:2013-02-15 Published:2017-12-29
  • Contact: Hua-Juan LIN E-mail:huajuanlin@vip.163.com

摘要: 金花茶粗多糖具有明显的降血脂作用,但是对于金花茶多糖的化学结构尚不清楚。金花茶粗多糖经过离子交换柱层析和凝胶柱层析法得到了分子质量为4.15×106u的多糖单一成分(TPS3-1),本文通过构成糖分析、红外光谱(IR)分析、核磁共振(NMR)分析以及阶梯式部分酸水解分析等手段对其毛发区域的化学结构特征进行进一步分析。构成糖分析结果显示,TPS3-1中中性糖约为半乳糖醛酸的2倍,其中鼠李糖、阿拉伯糖、半乳糖与葡萄糖之比为1:4.2:3.0:0.4,另外,62.58%的半乳糖醛酸残基以甲酯化形式存在。阶梯式部分酸水解分析结果显示,约有73%的中性糖降解至0.01 Mol/L三氟乙酸溶液中,推测该分子片段主要由葡萄聚糖支链、阿拉伯聚糖支链和半乳聚糖支链组成。约5%的中性糖降解于0.05 Mol/L三氟乙酸溶液中,推测该分子片段主要由葡萄糖或半乳糖与阿拉伯糖交替连接的寡糖支链组成。约13%的中性糖降解于0.5 Mol/L三氟乙酸溶液中,该分子片段主要由半乳糖和阿拉伯糖交替连接的寡糖支链以及零散分散于聚半乳糖醛酸链上的阿拉伯糖残基组成。

Abstract: Hypolipidaemic active was verified to be one of bioactive functions for crude polysaccharide from Camellia chrysantha (Hu) Tuyama, however, little is known about their chemical character. In this paper, a purified polysaccharide (Named TPS3-1) with molecular weight of 4.15×106u was obtained by ion-exchange chromatography and gel filtration chromatography, and the chemical character of its hairy region was studied by analysis of sugar composition, infrared spectrum (IR), nuclear magnetic resonance (NMR) and partially acidic hydrolysis by steps. The result of sugar composition analysis showed that the content of neutral sugar was about 2 fold to that of galacturonic acid (GalA), in which the ratio of rhamnose (Rha), alabinose (Ala) and galactose (Gal) was 1:4.2:3.0:0.4. In addition, 62.58% of GalAs existed as methyl esterified form. The result of partially acidic hydrolysis showed that approximately 73% of the neutral sugars were released to the solution of 0.01 Mol/L trifluoroacetic acid (TFA), and the molecular fragment was suggested to be consisted of branch chains including glucan, alabinan and galactan. 5% of the neutral sugars were released to the solution of 0.05 Mol/L TFA, which molecular fragment was suggested to be some oligosaccharides as branch chains, where alabinose may be alternately connected with glucose or galactose. 13% of the neutral sugars were released to the solution of 0.5 Mol/L TFA, which molecular fragment was suggested to be some oligosaccharides as branch chains, where some alabinoses may be alternately connected to galactose, and some mono alabinoses scarcely attached to the homogalacturonan bond chain.