食品科学 ›› 2013, Vol. 34 ›› Issue (5): 213-217.doi: 10.7506/spkx1002-6630-201305044

• 营养卫生 • 上一篇    下一篇

嗜酸乳杆菌NCFM胞外多糖对小鼠免疫相关基因的调控

杨相宜1,满朝新2,刘 颖1,王今雨1,郎 友1,董鑫悦1,闫天文1,姜毓君1,2,*   

  1. 1.东北农业大学食品学院 乳品科学教育部重点实验室,黑龙江 哈尔滨 150030; 2.东北农业大学 国家乳业工程技术研究中心,黑龙江 哈尔滨 150086
  • 收稿日期:2012-10-10 修回日期:2013-01-19 出版日期:2013-03-15 发布日期:2013-04-16
  • 通讯作者: 姜毓君 E-mail:yujun_jiang@163.com
  • 基金资助:
    国家自然科学基金;黑龙江省教育厅科学技术研究重点项目;国家“863计划”课题;长江学者和创新团队发展计划;东北农业大学博士启动基金

Regulation of Exopolysaccharide Produced by Lactobacillus acidophilus NCFM on Immune-Related Genes in Mice

YANG Xiang-yi1,MAN Chao-xin2,LIU Ying1,WANG Jin-yu1,LANG You1,DONG Xin-yue1,YAN Tian-wen1,JIANG Yu-jun1, 2,*   

  1. 1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China;2. National Research Center of Dairy Engineering and Technology, Northeast Agricultural University, Harbin 150086, China
  • Received:2012-10-10 Revised:2013-01-19 Online:2013-03-15 Published:2013-04-16
  • Contact: JIANG Yu-jun E-mail:yujun_jiang@163.com

摘要: 目的:研究嗜酸乳杆菌(Lactobacillus acidophilus)NCFM胞外多糖对小鼠免疫相关基因的调控作用。方法:通过构建小鼠模型,对小鼠脏器系数进行分析,提取肠组织RNA,采用Real-time RT-PCR方法对免疫相关基因chemokine (C-C motif) ligand 2(CCL2)和interleukin 18(IL18)的表达情况进行分析。结果:脏器系数随着天数的增加而增加,实验组的脏器系数较对照组相比,显著增加。在2个免疫相关基因中,随着时间的延长,IL18和CCL2呈现出先迅速增加后减少的趋势,同时均在第4天表达量达到最大值,且与对照组相比差异极显著(P<0.01)。结论:胞外多糖可以提高小鼠免疫脏器的质量,并且能够引起免疫相关基因出现特定表达变化,对小鼠机体的免疫调控具有一定的作用。

关键词: 嗜酸乳杆菌NCFM, 胞外多糖(EPS), 脏器系数, 免疫相关基因

Abstract: Objective: Exopolysaccharide (EPS) is a metabolic product of probiotics with antitumor, immunomodulatory and antioxidant functions. The aim of this study is to explore the effect of EPS produced by Lactobacillus acidophilus NCFM on immune-related genes in mice. Methods: Viscera coefficients were analyzed through mouse model. Real-time PCR was used to evaluate the expression of immune-related genes in mice after extracting total RNA from intestinal tissues. Results: The results indicated that the viscera coefficients revealed an increase with the extension of time and a significant difference in viscera coefficients between experimental group and control group (P<0.01). The expression of immune-related genes such as CCL2 and IL18 genes exhibited an initial increase and a final decrease with the extension of time and had the highest level on the 4th day. Similarly, a significant difference in the expression of these genes between experimental group and control group was observed (P<0.001). Conclusion: EPS can increase the relative weight of immune viscera, and affect the differential expression of immune-related genes. EPS produced by L. acidophilus NCFM can enhance immune function.

Key words: Lactobacillus acidophilus NCFM, exopolysaccharide (EPS), viscera coefficient, immune-related gene

中图分类号: