蛹虫草胞外多糖对MLD-STZ诱导的糖尿病小鼠降血糖机理的研究

doi:10.7506/spkx1002-6630-201311062

摘要/Abstract

摘要：

以链脲佐菌素诱导的糖尿病小鼠为实验模型，考察蛹虫草子实体粗多糖(BP)、菌丝体粗多糖(MP)以及胞外粗多糖(EP)的降血糖活性。结果显示只有EP具有显著的降血糖活性。进一步分别对糖尿病组、EP组的小鼠，以刀豆蛋白A刺激脾脏细胞检测其IL-4和IFN-γ水平，以脂多糖刺激脾脏巨噬细胞检测其IL-6和TNF-α水平。结果表明：与糖尿病组相比，EP组IL-4、IFN-γ水平及IFN-γ/IL-4均显著降低，而IL-6水平显著增加，从而导致TNF-α/IL-6比值降低，该结果与EP体外实验结果相符。因此，EP降血糖的免疫学机制可能在于通过提高脾脏巨噬细胞IL-6的水平诱导Th细胞向Th2细胞的分化，从而抑制糖尿病的发病进程。

关键词: 蛹虫草, 细胞因子, 多糖, 1型糖尿病

Abstract:

Cordyceps militaris, a Chinese herbal medicine with fruiting body or cultured mycelia, has been proposed
to have multiple medicinal activities. The aim of this study is to investigate the hypoglycemic activity of the crude
polysaccharides, extracted from fruiting body (BP), cultured mycelia(MP) and exopolysaccaride(EP), in diabetic mice
induced by multiple low-dose streptozotocin (MLD-STZ). Only EP showed potent hypoglycemic activity in diabetic
mice during the observing period, and this treatment group was examined immunologically. The proliferative response
of the spleen cells stimulated with Concanavalin A(ConA), the IL-4 and IFN-γ levels of the supernatant in stimulated
splenocytes, and the IL-6 and TNF-α levels secreted from the spleen macrophage stimulated with LPS were compared
among the control group, the EP group and the untreated diabetic group. Meanwhile, the proliferative response of the
lymphocytes and the related cytokines levels were also investigated with different concentrations of EP in vitro. Our
results showed that both the blastogenesis response and cytokines levels (IL-4 and IFN-γ) of splenocytes stimulated
by ConA were decreased in EP group, meanwhile the IFN-γ/IL-4 ratio was moderately inhibited. IL-6 secretion of the
splenic macrophage of the EP group was also significantly enhanced leading to a lower TNF-α/IL-6 ratio than that of the
diabetic mice. These data suggest that EP treatment could reduce the blood glucose in MLD-STZ induced diabetic mice.
The immunological basis of the hypoglycemic activity was probably associated with inhibiting the lymphocytes response
and inducing the differentiation of Th cells into Th2 cells through enhancement of IL-6 production by the splenic
macrophages.

Key words: Cordyceps militraris, cytokines, polysaccharide, type 1 diabetes

中图分类号:

孙纳新，杨桂文，安立国. 蛹虫草胞外多糖对MLD-STZ诱导的糖尿病小鼠降血糖机理的研究[J]. 食品科学, 2013, 34(11): 288-295.

SUN Na-xin，YANG Gui-wen，AN Li-guo. Anti-hyperglycemic Activities of Exopolysaccharides from Cordyceps militaris in Diabetic Mice Induced by Multiple Low-dose Streptozotocoin[J]. FOOD SCIENCE, 2013, 34(11): 288-295.

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