北虫草固态发酵产虫草素培养基优化

doi:10.7506/spkx1002-6630-201321051

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 254-258.doi: 10.7506/spkx1002-6630-201321051

北虫草固态发酵产虫草素培养基优化

顾冬艳，尹永祺，杨润强，顾振新*

南京农业大学食品科技学院，江苏南京 210095

收稿日期:2012-12-02 修回日期:2013-10-14 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 顾振新 E-mail:guzx@njau.edu.cn
基金资助:
江苏高校优势学科建设工程项目

Optimization of Medium Constituents for Cordycepin Production by Cordyceps militaris in Solid State Fermentation

GU Dong-yan，YIN Yong-qi，YANG Run-qiang，GU Zhen-xin*

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2012-12-02 Revised:2013-10-14 Online:2013-11-15 Published:2013-10-28
Contact: Zhenxin Gu E-mail:guzx@njau.edu.cn

摘要/Abstract

摘要：

优化北虫草固态发酵培养基以提高菌丝体中虫草素产量。通过单因素试验和正交试验，以虫草素产量为指标，研究培养基基质、液料比、营养液中碳源、氮源和金属离子对虫草素产量的影响并对其进行优化。结果表明：北虫草固态发酵的最佳培养基组成为大豆粉与麦麸质量比4:1、液料比39:30(V/m)及营养液中葡萄糖10g/L、牛肉膏2g/L、硫酸锰0.3g/L，在此条件下虫草素产量达5499.12μg/g。

关键词: 虫草素, 北虫草, 固态发酵, 培养基

Abstract:

The medium constituents for producing celium cordycepin by Cordyceps militaris in solid state fermentation
were optimized. The effects of medium matrix, liquid/solid ratio, carbon sources, nitrogen sources and metal ions in nutrient
solution on the yield of cordycepin were studied by single factor and orthogonal array design methods. Results suggested
that the optimal medium constituents were soybean meal/wheat bran ratio of 4:1, liquid/solid ratio of 39:30, glucose
concentration of 10 g/L, beef extract concentration of 2 g/L and manganese sulfate of 0.3 g/L in nutrient solution. Under
these conditions the yield of cordycepin was 5499.12 μg/g.

Key words: cordycepin, Cordyceps militaris, solid state fermentation, medium

中图分类号:

TS264.2

顾冬艳，尹永祺，杨润强，顾振新*. 北虫草固态发酵产虫草素培养基优化[J]. 食品科学, 2013, 34(21): 254-258.

GU Dong-yan，YIN Yong-qi，YANG Run-qiang，GU Zhen-xin*. Optimization of Medium Constituents for Cordycepin Production by Cordyceps militaris in Solid State Fermentation[J]. FOOD SCIENCE, 2013, 34(21): 254-258.

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北虫草固态发酵产虫草素培养基优化

Optimization of Medium Constituents for Cordycepin Production by Cordyceps militaris in Solid State Fermentation

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