食品科学 ›› 2013, Vol. 34 ›› Issue (22): 193-197.doi: 10.7506/spkx1002-6630-201322039

• 分析检测 • 上一篇    下一篇

贝类水产品中汞的形态分析

曹程明,冷桃花,解 楠,宁啸骏,周 瑶   

  1. 1.上海市质量监督检验技术研究院,国家食品质量监督检验中心(上海),上海 200233;
    2.上海市质量技术监督局,上海 200233;3.华东理工大学精细化工研究所,上海 200237
  • 收稿日期:2012-12-08 修回日期:2013-10-09 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 冷桃花 E-mail:length@sqi.org.cn
  • 基金资助:

    贝类水产品及其制品中汞的形态分析及风险监控研究

Speciation Analysis of Mercury in Shellfish Muscle

CAO Cheng-ming,LENG Tao-hua,XIE Nan,NING Xiao-jun,ZHOU Yao   

  1. 1. National Food Quality Supervision and Inspection Center (Shanghai), Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China;2. Shanghai Municipal Bureau of Quality and Technical Supervision, Shanghai 200233,China;3. Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, China
  • Received:2012-12-08 Revised:2013-10-09 Online:2013-11-25 Published:2013-12-05
  • Contact: Tao-Hua LENG E-mail:length@sqi.org.cn

摘要:

建立贝类水产品中二价汞、甲基汞、乙基汞和苯基汞的高效液相色谱-电感耦合等离子体质谱(HPLC-ICPMS)分离检测方法。本方法利用C18反相高效液相色谱柱对贝类水产品中二价汞、甲基汞、乙基汞和苯基汞进行分离,采用电感耦合等离子体质谱对汞元素进行检测,并利用外标法定量测定贝类水产品中各形态的汞含量。本研究优化色谱分离条件,以甲醇-乙酸铵溶液为流动相,通过梯度洗脱,4种汞形态在15min内得到了有效地分离。本方法操作简便、分离效果好、检出限低,适用于贝类水产品中有机汞的检测。

关键词: 二价汞, 甲基汞, 乙基汞, 苯基汞, 高效液相色谱-电感耦合等离子体质谱

Abstract:

Four mercury species (inorganic mercury, methyl-mercury, ethyl-mercury and phenyl-mercury) in shellfish muscle
were analyzed by high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). A
C18 reverse phase HPLC column was applied to separate four mercury species, and their concentrations were measured by using
ICP-MS through an external standard method. The method was optimized and the four mercury species could be effectively
separated within 15 min using gradient elution with methanol/ammonium acetate as the mobile phase. This developed method is
simple, efficient and sensitive, which can be suitable for determination of mercury species in shellfish muscle.

Key words: inorganic mercury, methyl-mercury, ethyl-mercury, phenyl-mercury, high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS)

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