食品科学 ›› 2013, Vol. 34 ›› Issue (22): 273-276.doi: 10.7506/spkx1002-6630-201322055

• 分析检测 • 上一篇    下一篇

羊胎盘免疫活性小分子肽的分离纯化

刘隆兴,任兴宏,汤禾静,唐照勇,何成明,方廖琼   

  1. 1.西南大学生物技术学院,重庆 400715;2.重庆理工大学药学与生物工程学院,重庆 400054
  • 收稿日期:2012-11-25 修回日期:2013-10-13 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 刘隆兴 E-mail:undefeatable@163.com

Separation and Purification of Small-Molecule Peptides with Immunomodulating Activity from Goat Placenta

LIU Long-xing,REN Xing-hong,TANG He-jing,TANG Zhao-yong,HE Cheng-ming,FANG Liao-qiong   

  1. 1. College of Biotechnology, Southwest University, Chongqing 400715, China;
    2. School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China
  • Received:2012-11-25 Revised:2013-10-13 Online:2013-11-25 Published:2013-12-05

摘要:

目的:从羊胎盘中分离纯化出具有免疫活性的小分子肽。方法:采用常规方法制备羊胎盘超滤液,利用反相高效液相色谱仪(RP-HPLC)对羊胎盘提取液进行分离纯化及纯度分析,并利用E-玫瑰花环实验对各组分进行免疫活性评价,利用Edman降解法对其组分进行N端测序。结果:利用RP-HPLC工艺成功分离出纯度较高的3种小分子肽,并确定其中两者具有免疫活性;对活性较强的肽进行测序、检索,结果表明为未知组分。结论:RP-HPLC是分离、分析活性肽类较理想的手段之一,并证实了羊胎盘含有具有免疫活性的小分子肽。

关键词: 羊胎盘小分子肽, 反相高效液相色谱, E-玫瑰花环

Abstract:

The purpose of this study was to isolate and purify small-molecule peptides with immunomodulating activity
from goat placenta. Ultrafiltration fraction from goat placenta was prepared by a conventional method, followed by
isolation, purification and analysis by reversed phase-high performance liquid chromatography (RT-HPLC). The immunomodulating
activity of its components was measured by E-rosette test and the N-terminal sequence was measured
by Edman degradation reaction. Three small-molecule peptides with high purity were obtained, two of which were immune-
active. The results of sequencing and retrieving showed that they were new peptides. RT-HPLC was one of the
optimal methods to purify and analyze bioactive peptides. The small-molecule peptides from goat placenta revealed
strong immune activity.

Key words: goat placenta small-molecule peptides, reversed phase-high performance liquid chromatography (RT-HPLC), E-rosette

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