海带多糖对大鼠血管平滑肌细胞凋亡的影响

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 344-348.

海带多糖对大鼠血管平滑肌细胞凋亡的影响

彭臻菲1,2，方哲翔1，刘敏1，陈立1，张其清1,*

福州大学

收稿日期:2012-11-21 修回日期:2013-09-25 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 彭臻菲 E-mail:bioenglab@163.com
基金资助:
福建省科技重大专项专题;福州大学科研启动基金

Apoptosis induction on rat vascular smooth muscle cells of Laminaria japonica polysaccharide

PENG Zhen-fei1,2，FANG Zhe-xiang1，LIU Min1，CHEN Li1，ZHANG Qi-qing1,*

Received:2012-11-21 Revised:2013-09-25 Online:2013-11-15 Published:2013-10-28
Contact: Zhen-fei PENG E-mail:bioenglab@163.com

摘要/Abstract

摘要：

目的：研究海带多糖诱导血管平滑肌细胞凋亡及其调控作用及其作用机制。方法：在碱性成纤维细胞生长因子诱导血管平滑肌细胞增殖模型基础上，运用MTT法考察海带多糖对VSMC细胞增殖的抑制作用；利用荧光倒置显微镜观察血管平滑肌细胞形态学的变化；通过流式细胞术分析血管平滑肌细胞周期的分布；采用Western-blot免疫印迹法探讨血管平滑肌细胞凋亡相关蛋白Bax和Bcl-2的表达。结果：海带多糖对血管平滑肌细胞增殖抑制呈量效相关性。细胞形态学观察结果及流式、Western-blot免疫印记分析结果表明海带多糖可诱导血管平滑肌细胞凋亡。其凋亡机制与其诱导细胞周期阻滞于S期以及提高Bax/Bcl-2比率有关。结论：海带多糖可诱导平滑肌细胞凋亡。

Abstract:

Purpose: To study the mechanism of apoptosis induced by Laminaria japonica polysaccharide. Methods: The proliferation of vascular smooth muscle cells induced by basic fibroblast growth factor (bFGF) was evaluated using MTT assay; The morphological changes of vascular smooth muscle cells were investigated by fluorescence microscope; The cell cycle distribution of vascular smooth muscle cells was determined using flow cytometry; The apoptotic factors were evaluated by Western-blot assay. Results: Laminaria japonica polysaccharide exhibited anti-proliferative activity on vascular smooth muscle cells in a dose-dependent manner. Further investigation indicated that Laminaria japonica polysaccharide induced vascular smooth muscle cells apoptosis. The apoptotic mechanism was related to the induction of S phase arrest, as well as regulating Bcl-2 family proteins expressions. Conclusions: Laminaria japonica polysaccharide could induce apoptosis.

中图分类号:

Q539

彭臻菲1,2，方哲翔1，刘敏1，陈立1，张其清1,*. 海带多糖对大鼠血管平滑肌细胞凋亡的影响[J]. 食品科学, 2013, 34(21): 344-348.

PENG Zhen-fei1,2，FANG Zhe-xiang1，LIU Min1，CHEN Li1，ZHANG Qi-qing1,*. Apoptosis induction on rat vascular smooth muscle cells of Laminaria japonica polysaccharide[J]. FOOD SCIENCE, 2013, 34(21): 344-348.

参考文献

[1] Liuzzo G. Atherosclerosis: an inflammatory disease. Rays,2001,26(4):221-230.
[2] Choy, J.C., Granville, D.J., Hunt, D.W.C., et al. Endothelial Cell Apoptosis: Biochemical Characteristics and Potential Implications for Atherosclerosis. J Mol Cell Cardiol, 2001, 33: 1673-1690.
[3] Yokoyama, M. Oxidant stress and atherosclerosis. Current Opinion in Pharmacology, 2004, 4:110–115.
[4] 耿雷, 刘闺男. 碱性成纤维细胞生长因子在烟草烟雾诱导大鼠血管平滑肌细胞增殖中的作用. 中国动脉硬化杂志, 2010, 18(5):345-350.
[5] Zhao, X., Xue, C.H., Li, B.F. Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica. J Appl Phycol, 2008, 20: 431-436.
[6] 王庭欣, 赵文, 蒋东升, 等. 海带多糖对糖尿病小鼠血糖的调节作用. 营养学报, 2001, 23 (2): 137-139.
[7] Wang, J., Zhang, Q., Zhang, Z., et al. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 2010, 46 (1): 6-12.
[8] 谢露, 王丽萍, 陈蒙华, 等. 海带多糖对肾上腺素致内皮细胞超微结构损害的保护作用. 中国应用生理学杂志,2010, 26 (2):232-236.
[9] 刘敏, 彭臻菲, 方哲翔, 等. 海带多糖硫酸酯对bFGF诱导的血管平滑肌细胞增殖的影响食品科学, 2012.
[10] D. M. Ornitz, N. Itoh. Fibroblast growth factors [J]. Genome Biol, 2001, 2(3): 1-12.
[11] C. B.Xu, Y. Zhang, E. Stenman, et al. D-erythro-N,N-dimethylsphingosine inhibits bFGF-induced proliferation of cerebral, aortic and coronary smooth muscle cells [J]. Atherosclerosis, 2002, 164: 237-243.
[12] R. Danesi, S. D. Bianchi, P. Soldani, et al. Suramin inhibits bFGF-induced endothelial cell proliferation and angiogenesis in the chick chorioallantoic membrane [J]. British Journal of Cancer, 1993, 68: 932-938.
[13] Peng, Z., Liu, M., Fang, Z., et al. In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica. International Journal of Biological Macromolecules, 2012, 50: 1254 -1259.
[14] 姬媛媛, 刘俊田, 王志东. 中药防治动脉粥样硬化研究现状评述. 中国实验方剂学杂志, 2006; 12 (4) : 582-601.
[15] Zhao, Y., Xiao, W., Douglas, M., et al.. Suppression of mitogen-activated protein kinase phosphatase-1 (MKP-1) by heparin in vascular smooth muscle cells. Biochemical Pharmacology, 2003, 66: 769–776.
[16] Suh, S.J., Moon, S.K., Kim, C.H. Raphanus sativus and its isothiocyanates inhibit vascular smooth muscle cells proliferation and induce G1 cell cycle arrest. International Immunopharmacology, 2006, 6: 854–861.
[17] 甄艳军, 王林, 赵兰英, 等. 两种木贼提取物对动脉粥样硬化早期模型大鼠平滑肌凋亡及Bcl-2、Cas pase-3表达的调控 [J]. 中国老年学杂志, 2007, 27 (4): 308-309.