食品科学 ›› 2014, Vol. 35 ›› Issue (1): 239-243.doi: 10.7506/spkx1002-6630-201323047

• 营养卫生 • 上一篇    下一篇

RS4型抗性淀粉对高脂饮食C57BL/6J小鼠炎症因子的影响

王 欣,邬应龙*   

  1. 四川农业大学食品学院,四川 雅安 625014
  • 收稿日期:2013-01-09 修回日期:2013-11-26 出版日期:2014-01-15 发布日期:2014-01-22
  • 通讯作者: 邬应龙 E-mail:wuyinglong99@163.com
  • 基金资助:

    四川省科技支撑计划项目(2009NZ0077-007);四川农业大学“211”工程双支计划项目(2010)

Effect of RS4-Type Resistant Starch on Inflammatory Cytokines in C57BL/6J Mice Fed High-Fat Diet

WANG Xin, WU Ying-long*   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2013-01-09 Revised:2013-11-26 Online:2014-01-15 Published:2014-01-22
  • Contact: Yinglong Wu E-mail:wuyinglong99@163.com

摘要:

目的:研究3种RS4型抗性淀粉对高脂饮食C57BL/6J小鼠炎症因子的影响。方法:雄性C57BL/6J小鼠随机分成6组:基础对照(control,CL)组、高脂饮食(high-fat,HF)组及高脂饲料中分别添加15%甘薯淀粉(sweet potatostarch,SPS)、羟丙基甘薯淀粉磷酸酯(hydroxypropyl cross-linked sweet-potato modified starch,HPCL-SPS)、交联辛烯基琥珀酸甘薯淀粉酯(cross-linked octenyl succinic anhydride sweet-potato modified starch,CLOSA-SPS)、柠檬酸乙酰化甘薯淀粉酯(citric acid acetylated sweet-potato modified starch,CAAC-SPS)饲喂12周,处死后测定血清中游离脂肪酸(free fatty acid,FFA)、肿瘤坏死因子α(necrosis factor-α,TNF-α)、白介素6(interlukin 6,IL-6)、单核细胞趋化蛋白1(monocyte-chemoattractant protein 1,MCP-1)等指标。结果:HF组和HF-SPS组的FFA、TNF-α、IL-6和MCP-1水平明显高于CL组,HF-HPCL-SPS组、HF-CAAC-SPS组的FFA、TNF-α、IL-6和MCP-1的含量及附睾周围脂肪大小显著低于HF组(P<0.05)。结论:HPCL-SPS、CAAC-SPS可以降低高脂饮食C57BL/6J小鼠血清中FFA和炎症因子水平,并影响脂肪细胞的大小,可能对由肥胖引起的低度炎症及相关代谢疾病有一定的改善作用。

关键词: RS4型抗性淀粉, 肥胖, 炎症因子, 脂肪细胞

Abstract:

Objective: To analyze the effects of three kinds of RS4 resistant starch on inflammatory cytokines in C57/BL6J
mice fed high-fat diet. Methods: A total of 72 C57BL/6J male mice were randomly divided into six groups with 12 mice in
each group: control group (CL), high-fat group (HF) and HF-SPS, HF-HPCL-SPS, HF-CLOSA-SPS and HF-CAAC-SPS
groups, fed a high fat diet containing 15% sweet potato starch (SPS), hydroxypropyl cross-linked sweet-potato modified
starch (HPCL-SPS), cross-linked octenyl succinic anhydride sweet-potato modified starch (CLOSA-SPS) and citric acid
acetylated sweet-potato modified starch (CAAC-SPS), respectively. After 12 weeks of feeding, the levels of serum free fatty
acid (FFA), necrosis factor-α(TNF-α), interlukin6 (IL-6) and monocyte-chemoattractant protein 1 (MCP-1) were determined.
Results: The levels of serum FFA, TNF-α, IL-6 and MCP-1 of HF group and HF-SPS group were significantly higher than
those in CL group. The size of epididymal adipocyte and the levels of serum FFA, TNF-α, IL-6 and MCP-1 were reduced
by HPCL-SPS and CAAC-SPS treatments. Conclusions: HPCL-SPS and CAAC-SPS can suppress the serum FFA and
inflammatory cytokines and affect the size of epididymal adipocyte in C57BL/6J mice fed high-fat diet, and thus, have an
improvement on obesity-related low-grade inflammatory state and metabolic diseases.

Key words: RS4-type resistant starch, obesity, inflammatory cytokines, adipocyte

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