食品科学 ›› 2013, Vol. 34 ›› Issue (9): 358-363.doi: 10.7506/spkx1002-6630-201309071

• 专题论述 • 上一篇    下一篇

功能性低聚糖合成中糖基转移酶研究进展

李 玉,路福平,王正祥   

  1. 工业发酵微生物教育部重点实验室,工业酶国家工程实验室,天津科技大学生物工程学院,天津 300457
  • 收稿日期:2013-03-26 修回日期:2013-04-20 出版日期:2013-05-15 发布日期:2013-05-07
  • 通讯作者: 李玉 E-mail:liyu@tust.edu.cn
  • 基金资助:

    国家“863”计划项目(2013AA102106);“十二五”农村领域国家科技计划课题(2011AA100905-4);
    教育部“长江学者和创新团队发展计划”资助项目(IRT1166)

Recent Advance in Research and Application of Several Glycosyltransferases in the Synthesis of Functional Oligosaccharides

LI Yu,LU Fu-ping,WANG Zheng-xiang   

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, National Engineering Laboratory for Industrial
    Enzymes, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
  • Received:2013-03-26 Revised:2013-04-20 Online:2013-05-15 Published:2013-05-07
  • Contact: LI Yu E-mail:liyu@tust.edu.cn

摘要:

低聚异麦芽糖、低聚果糖、岩藻糖基化寡糖和唾液酸化寡糖等功能性低聚糖因具有显著促双歧杆菌增殖的作用,已被广泛应用于婴幼儿乳粉及食品中,目前合成这些功能糖的主要途径是利用微生物所产的α-葡萄糖基转移酶、果糖基转移酶、岩藻糖基转移酶或唾液酸转移酶等糖基转移酶,以葡萄糖、蔗糖或乳糖等作为底物进行合成。本文针对这几类微生物糖基转移酶的来源、性质、活性、表达和在功能性低聚糖合成中的应用以及相关研究进行简要的概括和总结,为高活力糖基转移酶的研究与开发以及改善其在功能低聚糖生产中的应用性能提供一定的参考。

关键词: &alpha, -葡萄糖基转移酶;果糖基转移酶;岩藻糖基转移酶;唾液酸转移酶

Abstract:

Isomaltooligosacharide, fructooligosaccharides, fucosylated oligosaccharide and sialyloligosaccharide have
been widely applied in milk powder for infants and young children because of its special physiological effect to promote the
growth of bifidobacterium. These functional oligosaccharides are produced by α-glycosyltransferase, fructosyltransferase,
fucosyltransferase and sialyltransferase, using glucose, sucrose and lactose as substrates, respectively. This paper
reviews recent research advances in the microbial souces, properties, activity and expression of these enzymes and their
applications in the synthesis of functional oligosaccharides in order to provide refereces for the development of highly active
glycosyltransferases and the improvement of their applicability for the production of functional oligosaccharides.

Key words: &alpha, -glycosyltransferase;fructosyltransferase;fucosyltransferase;sialyltransferase

中图分类号: