食品科学 ›› 2013, Vol. 34 ›› Issue (9): 351-357.doi: 10.7506/spkx1002-6630-201309070

• 专题论述 • 上一篇    下一篇

微生物酶高效异源表达策略的最新研究进展

杨海泉,刘 龙,李江华,堵国成,陈 坚   

  1. 1.江南大学 工业生物技术教育部重点实验室,江苏 无锡 214122;2.江南大学生物工程学院,江苏 无锡 214122; 3.江南大学 粮食发酵工艺与技术国家工程实验室,江苏 无锡 214122
  • 收稿日期:2013-04-08 修回日期:2013-04-24 出版日期:2013-05-15 发布日期:2013-05-07
  • 通讯作者: 堵国成 E-mail:kegcdu@yahoo.com.cn

Recent advances in high-level heterologous expression of microbial enzymes

YANG Hai-quan,LIU Long,LI Jiang-hua,DU Guo-cheng,CHEN Jian   

  • Received:2013-04-08 Revised:2013-04-24 Online:2013-05-15 Published:2013-05-07
  • Contact: Guo-Cheng DU E-mail:kegcdu@yahoo.com.cn

摘要: 酶是一种高专一性的生物催化剂,在化工、农业、医药、食品、纺织、饲料等领域具有广泛应用。常见的酶表达宿主包括大肠杆菌、芽孢杆菌、丝状真菌、酵母、乳酸菌等。不同类型的表达宿主具有不同的特点,如细菌宿主可用来快速地过量表达原核来源的酶,但真核来源的大分子蛋白由于翻译后修饰的原因往往不能在细菌表达系统中正确表达,仅可以在真核表达宿主中正确表达。近年来利用不同表达宿主实现工业酶的高效异源表达取得了很大进展。本文系统总结了在不同表达宿主中实现工业酶高效异源表达策略的最新研究进展,并对工业酶高效异源表达技术的发展趋势进行了展望。

关键词: 酶, 异源表达, 大肠杆菌, 芽孢杆菌, 酵母, 丝状真菌, 乳酸菌

Abstract: Enzyme is a kind of biological catalyst with high specificity and has been widely used in chemical industry, agricultural industry, biopharmaceutical industry, food industry, textile industry, feed industry and other industries. The commonly used industrial enzyme expression hosts include Escherichia coli, Bacillus, filamentous fungi, yeast, and lactic acid bacteria. Different expression hosts have different characteristics. For example, the bacterium hosts can quickly and easily over-express recombinant enzymes, but the bacterium systems cannot express very large eukaryotic proteins because of proteins requiring post-translational modifications. In recent years, the great progresses have been made in the high-level expression of industrial enzymes in different hosts. In this work, we systematically summarize the recent advances in the heterologous expression of industrial enzymes, and the development trends of heterologous expression of industrial enzymes are also prospected.