用于亚硝酸盐快速检测的三维纸质微流控芯片的制作

doi:10.7506/spkx1002-6630-201322069

食品科学 ›› 2013, Vol. 34 ›› Issue (22): 341-345.doi: 10.7506/spkx1002-6630-201322069

用于亚硝酸盐快速检测的三维纸质微流控芯片的制作

肖良品，刘显明，刘启顺，钟润涛，周小棉，林炳承，杜昱光

1.中国科学院大连化学物理研究所，辽宁大连 116023；2.中国科学院大学，北京 100049；
3.芯片实验室研究中心(中国大连)，辽宁大连 116023；4.广州市第一人民医院，广东广州 510180

收稿日期:2013-01-30 修回日期:2013-10-29 出版日期:2013-11-25 发布日期:2013-12-05
通讯作者: 肖良品 E-mail:lpxiaodicp@foxmail.com

Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite

XIAO Liang-pin，LIU Xian-ming，LIU Qi-shun，ZHONG Run-tao，ZHOU Xiao-mian，LIN Bing-cheng，DU Yu-guang

1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China；2. University of Chinese Academy of Sciences, Beijing 100049, China；3. Research Centre of Laboratory on a Chip (Dalian China), Dalian 116023, China；4. Guangzhou First Municipal People’s Hospital, Guangzhou 510180, China

Received:2013-01-30 Revised:2013-10-29 Online:2013-11-25 Published:2013-12-05
Contact: Liangpin XIAO E-mail:lpxiaodicp@foxmail.com

摘要/Abstract

摘要：

选用中速定性滤纸作为纸芯片制作材料，采用装订法制作三维纸芯片，以63.4g/L柠檬酸溶液、8.61g/L对氨基苯磺酰胺、2.59g/L N-(1-萘基)乙二胺盐酸盐作为显色剂，结合实验室自制的比色检测装置用于亚硝酸盐的检测。结果表明：设计制作的三维纸质微流控芯片结合自制的比色检测装置，可以实现亚硝酸盐的快速定量检测，在0～10mg/L的质量浓度范围内有良好的线性，R2为0.9920，最低检测限为2mg/L，加标回收率为91.4%～102.0%，制作的亚硝酸盐检测纸芯片比较稳定，在室温条件下放置7周，得到的显色结果与新制作的纸芯片的显色结果基本一致。亚硝酸盐检测纸芯片简单易用、成本低、耗样量少、灵敏度较好，能快速实现亚硝酸盐的检测。

关键词: 纸质微流控芯片, 三维, 亚硝酸盐, 快速检测

Abstract:

Medium-speed qualitative filter paper was employed to fabricate paper-based microfluidics and 3D paper-based
microfluidics were integrated by wax printing-bookbinding method; a nitrite indicator containing 63.4 g/L citric Acid, 8.61 g/L sulfanilamide
and 2.59 g/L N-(1-naphthyl) ethylenediamine coupled with a lab-prepared colorimetric detection device was used
for nitrite determination. The 3D paper-based microfluidics could rapidly test nitrite, and the image acquired was analyzed
using a gray model for gray intensity representing the concentration of nitrite. The linear range was 0–10 mg/L, with correlation
coefficient of 0.9920. The detection limit of the method was 2 mg/L, and the recovery of nitrite from spiked samples
was 91.4%–102.0%. After stored under normal conditions and 4 ℃ for 7 weeks, the color intensity of the paper-based microfluidics
for nitrite detection were little change. The 3D paper-based microfluidics can provide a simple, easy to use, low-cost
and sensitive approach for rapid detection of in foods with small sample consumption.

Key words: paper-based microfluidics, three-dimension, nitrite, rapid detection

中图分类号:

肖良品，刘显明，刘启顺，钟润涛，周小棉，林炳承，杜昱光. 用于亚硝酸盐快速检测的三维纸质微流控芯片的制作[J]. 食品科学, 2013, 34(22): 341-345.

XIAO Liang-pin，LIU Xian-ming，LIU Qi-shun，ZHONG Run-tao，ZHOU Xiao-mian，LIN Bing-cheng，DU Yu-guang. Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite[J]. FOOD SCIENCE, 2013, 34(22): 341-345.

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用于亚硝酸盐快速检测的三维纸质微流控芯片的制作

Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite

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