食品科学

• 工艺技术 •    下一篇

酶法提取克氏原螯虾头和虾壳的中蛋白质

王 燕,邓放明*,刘 焱,廖 泉,刘文倩   

  1. 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,湖南 长沙 410128
  • 出版日期:2013-06-25 发布日期:2013-06-17

Enzymatic Extraction of Protein from Crayfish Head and Shell

WANG Yan,DENG Fang-ming*,LIU Yan,LIAO Quan,LIU Wen-qian   

  1. Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

采用蛋白酶法对克氏原螯虾头和虾壳中蛋白质的提取工艺进行研究。单因素试验和正交试验结果表明:虾头和虾壳中蛋白质提取的最优工艺条件为木瓜蛋白酶与风味蛋白酶按1:1.5混合作为复合蛋白酶,酶解温度为50℃、酶解时间为3h、蛋白酶用量为1.0%、pH6.5、料液比为1:10,该条件下蛋白质提取率为54.22%。

关键词: 克氏原螯虾头, 克氏原螯虾壳, 木瓜蛋白酶, 风味蛋白酶, 蛋白质

Abstract:

This paper describes the enzymatic extraction of protein from crayfish head and shell. Using one-factor-at-atime
and orthogonal array design, we established the optimum conditions for extracting protein from crayfish head and
shell as follows: 1:1.5 of papain/flavourzyme ratio with a total enzyme concentration of 1.0%, pH 6.5, 1:10 of solid/liquid
ratio, 50 ℃ and a hydrolysis duration of 3 h. The extraction efficiency of protein under the optimized conditions was 54.22%.

Key words: crayfish head, crayfish shell, papain, flavourzyme, protein