食品科学

• 工艺技术 • 上一篇    下一篇

糖化酶型时间-温度指示器反应体系的制备

郑光临,钱 静,冯 钦   

  1. 江南大学机械工程学院,江苏 无锡 214122
  • 出版日期:2013-06-25 发布日期:2013-06-17

Preparation of Time-Temperature Indicator Reaction System Based on Glucoamylase

ZHENG Guang-lin,QIAN Jing*,FENG Qin   

  1. School of Mechanical Engineering, Jiangnan University, Wuxi 214122, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

利用糖化酶与淀粉的水解反应,以碘液为指示剂,通过颜色变化可以显示时间和温度的累积效应。确定糖化酶型时间-温度指示器反应体系的参数为:以10mL质量浓度40g/L的麦芽糊精为反应底物,3mL质量浓度为1g/L的碘液,糖化酶的加入量是可变的。该时间-温度指示器可应用于冷链运输时间不同的产品。加酶量为50μL的反应体系适用于3~4d的4℃左右的低温温控包装中,通过测定其在不同温度条件下的反应速率,确定该反应体系的活化能为75.94kJ/mol。该体系可以应用于因脂肪氧化而造成质量损失的冷鲜肉等产品。

关键词: 时间-温度指示器, 糖化酶, 冷链运输, 活化能, 脂肪氧化

Abstract:

Color changes occuring during the course of reaction between glucoamylase and starch in the presence of iodine
solution as an indicator display the cumulative effects of time and temperature. A time-temperature indicator reaction
system was developed with 10 mL of 40 g/L maltodextrin as reaction substrate, 3 mL of 1 g/L iodine solution and variable
amounts of glucoamylase. The time-temperature indicator was widely applicable for products at different times of cold chain
transportation. The reaction system with 50 μL of glucoamylase was suitable for low temperature controlled packaging for
3 to 4 d at about 4 ℃, and the activation energy was found to be 75.94 kJ/mol. This system had potential application for
chilled fresh meat, which is vulnerable to quality deterioration caused by lipid peroxidation.

Key words: time-temperature indicator, glucoamylase, cold chain transportation, activation energy, lipid oxidation