食品科学

• 工艺技术 • 上一篇    下一篇

正交试验优化大豆多肽脱色工艺

戴丽君,梁运祥   

  1. 华中农业大学生命科学技术学院,农业微生物国家重点实验室,湖北 武汉 430070
  • 出版日期:2013-06-25 发布日期:2013-06-17

Optimization by Orthogonal Array Design of Decolorization of Soybean Peptide

DAI Li-jun,LIANG Yun-xiang   

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology,Huazhong Agricultural University, Wuhan 430070, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

对活性炭和AB-8型树脂分步处理大豆多肽的脱色工艺进行优化。以脱色率和肽损失率为指标,考察活性炭用量、脱色时间、脱色温度、脱色pH值对大豆多肽液脱色的影响,并通过正交试验进行优化。结果表明:活性炭脱色最佳条件为活性炭用量2.0%(m/m)、脱色时间50min、脱色温度55℃、pH8.0,此时大豆多肽液脱色率达到57.13%,肽损失率11.37%。用AB-8树脂(0.5g/mL)对活性炭脱色处理过的大豆肽液进行再次脱色处理,脱色9h后脱色率57.99%,肽损失率16.19%。两步脱色后的大豆多肽液总脱色率达到81.97%,肽总损失率26.74%。

关键词: 大豆多肽, 活性炭, AB-8树脂, 脱色

Abstract:

In this study, an optimized method was presented for the decolorization of soybean peptide by stepwise treatment
with activated carbon and AB-8 resin. An orthogonal array design was used to optimize activated carbon amount, time,
temperature and pH based on decolorization rate and peptide loss rate. The optimal conditions for activated carbon treatment
were determined to be decolorization at 55 ℃ for 50 min with the addition of 2.0% of activated carbon adjusted to pH
8.0, giving rise to a decolorization rate of 57.13% and a peptide loss of 11.37%. Further decolorization was performed by
9 h treatment with AB-8 resin (0.5 g/mL), resulting in a decolorization rate of 57.99% and a peptide loss of 16.19%. The
total decolorization rate and total peptide loss were 81.97% and 26.74%, respectively, after the two-stage decolorization.

Key words: soybean peptide, activated carbon, AB-8 resin, decolorization