食品科学

• 工艺技术 • 上一篇    下一篇

新疆琐琐葡萄叶总黄酮提取工艺及抗氧化活性

古丽巴哈尔·阿巴拜克力   

  1. 新疆师范大学生命科学学院,新疆 乌鲁木齐 830054
  • 出版日期:2013-06-25 发布日期:2013-06-17

Extraction and Antioxidant Activity of Total Flavonoids from Leaves of Vitis viniferal L. Grown in Xinjiang GULIBAHAER Ababaikeli

GULIBAHAER Ababaikeli   

  1. College of Life Science, Xinjiang Normal University, Ürümqi 830054, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

采用分光光度法,以芦丁为标准样品,测定琐琐葡萄叶中黄酮类化合物的含量。在考察各影响因素的基础上设计正交试验,得出各种因素对提取效果的影响主次顺序为提取时间>乙醇体积分数>温度>料液比。黄酮类化合物的最佳提取工艺为50%乙醇、提取时间100min、料液比1:50、提取温度70℃,在最佳提取条件下测定黄酮提取液的还原力、抗氧化能力,总黄酮对不同自由基的抑制能力由大到小依次为DPPH自由基>·OH>O2-·。

关键词: 琐琐葡萄叶, 总黄酮, 还原力, 抗氧化

Abstract:

The extraction of total flavonoids from leaves of Vitis viniferal L. was optimized by orthogonal array design with
the objective of developing a sample preparation method for the spectrophotometric determination of total flavonoids with rutin
as a standard reference material. Four extraction parameters were ranked in decreasing order of importance as follows: time,
ethanol concentration, temperature and solid-to-solvent ratio, and their optimal conditions were 100 min, 50%, 70 ℃ and 1:50,
respectively. The total flavonoids obtained under the optimized conditions possessed free radical scavanging activity against
DPPH, hydroxyl and superoxide anion radicals in decreasing order as well as reducing power.

Key words: Vitis viniferal L., total flavonoids, reduction power, antioxidant activity