食品科学

• 分析检测 • 上一篇    下一篇

HPLC-HG-AFS联用技术检测蜂花粉中砷形态

王素芬,陈 芳,王 鹏,吴黎明   

  1. 1.福建农林大学蜂学学院,福建 福州 350002;2.中国农业科学院蜜蜂研究所,北京 100093
  • 出版日期:2013-06-25 发布日期:2013-06-17

Determination of Four Arsenic Species in Bee Pollen by High Performance Liquid Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry

WANG Su-fen,CHEN Fang,WANG Peng,WU Li-ming   

  1. 1. College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

建立高效液相色谱-氢化物发生-原子荧光光谱(HPLC-HG-AFS)联用技术分析蜂花粉中亚砷酸(AsⅢ)、砷酸(AsⅤ)、一甲基砷酸(MMA)和二甲基砷酸(DMA)的方法。以0.20mol/L硫酸溶液为提取溶剂,经超声、离心提取,用0.22μm滤膜过滤后,经高效液相色谱-氢化物发生-原子荧光仪分离测定。结果表明:AsⅢ、DMA、MMA和AsⅤ的检测限分别是1.0、2.1、1.2、3.1μg/kg,加标回收率均在79%~100%之间,精密度实验结果(n=4)显示,4种砷形态的相对标准偏差分别为4.5%、2.3%、11.0%、7.7%。该方法快速准确、灵敏度高,是一种较好的定性和定量分析蜂花粉中不同形态砷的方法。

关键词: 蜂花粉, 砷形态, 高效液相色谱-氢化物发生-原子荧光仪

Abstract:

This paper describes a high performance liquid chromatography- hydride generation-atomic fluorescence
spectrometry (HPLC-HG-AFS) method for the determination of arsenite (AsⅢ), monomethylarsonic acid (MMA),
dimethylarsinic acid (DMA) and arsenate (AsⅤ) in bee pollen. Sample preparation was achieved by ultrasonic extraction
with 0.20 mol/L sulfuric acid. The detection limits for AsⅢ, DMA, MMA and AsⅤ were 1.0, 2.1, 1.2 and 3.1 μg/kg,
respectively, and recoveries at two spiked levels were in the range of 79%–100% with relative standard deviations (RSDs)
of 4.5%, 2.3%, 11.0% and 7.7%, respectively. In conclusion, we have established a good, fast qualitative and quantitative
method for analysis of arsenic compounds in bee pollen with high sensitivity and good accuracy.

Key words: bee pollen, arsenic species, high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS)