高效液相色谱-串联质谱法测定水果蔬菜中的乙萘酚

doi:10.7506/spkx1002-6630-201312044

食品科学

高效液相色谱-串联质谱法测定水果蔬菜中的乙萘酚

夏爽，郗存显，张雷，严爱花，唐柏彬，王国民，母昭德

1.重庆医科大学药学院，重庆 400016；
2.重庆出入境检验检疫局，重庆市进出口食品安全工程技术研究中心，重庆 400020

出版日期:2013-06-25 发布日期:2013-06-17

Determination of 2-Naphthol in Fruits and Vegetables by High Performance Liquid Chromatography-Tandem Mass Spectrometry

XIA Shuang，XI Cun-xian，ZHANG Lei，YAN Ai-hua，TANG Bo-bin，WANG Guo-min，MU Zhao-de

1. College of Pharmacy, Chongqing Medical University, Chongqing 400016, China；
2. Chongqing Engineering and Technology Research Center for Import and Export Food Safety,Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 400020, China

Online:2013-06-25 Published:2013-06-17

摘要/Abstract

摘要：

建立柑橘、苹果、菠菜、西红柿等水果和蔬菜中乙萘酚的高效液相色谱-串联质谱定量分析检测方法。样品采用乙腈提取，LC-NH2固相萃取柱净化，采用液相色谱-质谱联用技术-电喷雾离子源多反应监测模式检测，外标法定量。结果表明：乙萘酚在50.00～1000.00μg/L范围内线性关系良好(r2≥0.9997)，检出限为10μg/kg；在10、20μg/kg和50μg/kg三个添加水平下回收率范围在70.4%～105.5%之间，相对标准偏差(RSD)范围为4.1%～10.6%。

关键词: 高效液相色谱-串联质谱, 乙萘酚, 水果, 蔬菜

Abstract:

An analytical method based on high performance liquid chromatography-tandem mass spectrometry was
developed for the determination of 2-naphthol in fruits and vegetables. Samples were extracted with acetonitrile, and the
extract was then cleaned up on an LC-NH2 cartridge. The analytes were determined by LC-MS/MS with electrospray
ionization source in multiple reaction monitoring (MRM). Quantification was conducted by external standard calibration.
Good linearity was observed in the range of 50.00 to 1000.00 μg/L (r2 ≥ 0.9997). The limit of detection of the established
method was 10 μg/kg. The recovery rate of 2-naphthol was 70.4%–105.5% at spiked levels of 10, 20 μg/kg and 50 μg/kg in
deferent samples, with relative standard deviation of 4.1%–10.6%.

Key words: high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), 2-naphthol, fruits, vegetables

夏爽，郗存显，张雷，严爱花，唐柏彬，王国民，母昭德. 高效液相色谱-串联质谱法测定水果蔬菜中的乙萘酚[J]. 食品科学, doi: 10.7506/spkx1002-6630-201312044.

XIA Shuang，XI Cun-xian，ZHANG Lei，YAN Ai-hua，TANG Bo-bin，WANG Guo-min，MU Zhao-de. Determination of 2-Naphthol in Fruits and Vegetables by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201312044.

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高效液相色谱-串联质谱法测定水果蔬菜中的乙萘酚

Determination of 2-Naphthol in Fruits and Vegetables by High Performance Liquid Chromatography-Tandem Mass Spectrometry

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