食品科学

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定东盟水果中氟啶脲残留量

吕 平,杜国冬,韦丽君,农耀京   

  1. 1.广西壮族自治区亚热带作物研究所,广西 南宁 530001;
    2.农业部亚热带果品蔬菜质量监督检验测试中心,广西 南宁 530001
  • 出版日期:2013-06-25 发布日期:2013-06-17

Development of a High-Performance Liquid Chromatographic Method for Detection of Chlorfluazuron Residues in Fruits Cultivated in Southeast Asian

LÜ Ping,DU Guo-dong,WEI Li-jun,NONG Yao-jing   

  1. 1. Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops, Nanning 530001, China;
    2. Quality Supervision and Testing Center of Subtropical Fruit and Vegetable, Ministry of Agriculture, Nanning 530001, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

建立东盟水果中氟啶脲残留的检测方法,以香蕉、荔枝和芒果为材料,利用高效液相色谱法对东盟水果中氟啶脲进行研究。采用丙酮提取样品,提取液经弗罗里硅土柱净化,用高效液相色谱仪结合紫外检测器进行测定,外标法定量。结果表明:氟啶脲在0.05~5.00mg/L质量浓度范围内线性关系良好,相关系数为0.99996;该方法的添加回收率在84.7%~102%之间,变异系数在2.0%~6.7%之间,最低定量限为0.02mg/kg。建立的氟啶脲残留量测定方法满足国家标准对食品中农药残留测定的要求,可用于相关农产品及食品中苯酰脲类农药的质量监控。

关键词: 氟啶脲, 高效液相色谱法, 水果

Abstract:

A analytical method was established to determine chlorfluazuron residues in banana, mango and litchi
cultivated in Southeast Asia by high performance liquid chromatography (HPLC). Samples were extracted acetone
and the extact was then cleaned up on a Florisil column prior to analysis by HPLC with UV detection. The analytical
column was an Agilent Eclip se XDB C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was methyl alcoholwater
(90:10, by volume) at a flow rate of 1.0 mL/min. The detection wavelength was set at 258 nm. Quantification was
performed by the external standard method. The results showed that the linear range for chlorfluazuron was in the range
of 0.05-5.00 mg/L with a correlation coefficient of 0.99996. The average recoveries of chlorfluazuron were between 84.7%
and 102%, with a variation coefficient ranging from 2.0% to 6.7%. The limit of quantification of this method was 0.02 mg/kg.
This method met the national requirements for pesticide determination and was applicable to food quality supervision and
control on chlorfluazuron.

Key words: chlorfluazuron, high performance liquid chromatography, fruits