食品科学

• 分析检测 • 上一篇    下一篇

碳纳米管修饰的过氧化苯甲酰生物传感器的研制

丁建英,潘欢欢,张根华,权 英,詹月华   

  1. 常熟理工学院生物与食品工程学院,江苏 常熟 215500
  • 出版日期:2013-06-25 发布日期:2013-06-17

Frabrication of Benzoyl Peroxide Biosensor Based on Carbon Nanotube-Modified Electrode

DING Jian-ying,PAN Huan-huan,ZHANG Gen-hua,QUAN Ying,ZHAN Yue-hua   

  1. College of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

构建一种用于检测面粉中过氧化苯甲酰(BPO)的电化学酶传感器。将碳纳米管作为电子介体修饰于玻碳电极表面,用溶胶凝胶将辣根过氧化物酶固定在碳纳米管修饰的电极上,利用辣根过氧化物酶对BPO的催化引起电极电流响应的变化,制成新型的BPO生物传感器。结果表明:在BPO浓度为8.3×10-6~3.4×10-3mol/L范围内,酶电极响应电流的变化值与BPO的浓度呈良好的线性关系,最低检出限为2.3×10-6mol/L。该传感器具有灵敏、稳定、易于制备的优点,有望用于面粉中BPO的快速检测。

关键词: 过氧化苯甲酰, 电化学酶传感器, 碳纳米管, 玻碳电极

Abstract:

An electrochemical enzyme biosensor was developed to determine benzoyl peroxide (BPO) in flour. Carbon
nanotube was modified on the surface of glassy carbon electrode as an electron mediator, followed by coating the electrode
surface with horseradish peroxidase embedded in chitosan sol-gel. A new type of benzoyl peroxide biosensor was fabricated
based on changes in the electrode current response caused by the catalytic action of horseradish peroxidase on BPO. The results
showed that the response current of the sensor was linearly related to BPO concentration over the range of 8.3 × 10-6 mol/L
to 3.4 × 10-3 mol/L with a detection limit of 2.3 × 10-6 mol/L. This biosensor is sensitive, fast and convenient and has the
potential to be applied for the determination of benzoyl peroxide in flour.

Key words: benzoyl peroxide, electrochemical enzyme sensor, carbon nanotube, glassy carbon electrode