食品科学

• 分析检测 • 上一篇    下一篇

HPLC法测定茯茶中黄酮类成分

李 适,卢 强,李银花,黄建安,刘仲华   

  1. 1.湖南农业大学植物资源工程系,湖南 长沙 410128;2.湖南农业大学 茶学教育部重点实验室,湖南 长沙 410128
  • 出版日期:2013-06-25 发布日期:2013-06-17

Determination of Flavonoids in Fuzhuan Tea by HPLC

LI Shi,LU Qiang,LI Yin-hua,HUANG Jian-an,LIU Zhong-hua   

  1. 1. Department of Botanic Resource Engineering, Hunan Agricultural University, Changsha 410128, China;
    2. Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China
  • Online:2013-06-25 Published:2013-06-17

摘要:

目的: 建立茯茶中的黄酮类成分的高效液相色谱(HP LC) 分析测定方法。方法:We l c h r om C18(250mm×4.6mm,5μm)色谱柱,流速0.8mL/min,检测波长295nm,分析时间80min;以A相甲醇、B相0.5%冰醋酸为洗脱溶剂梯度洗脱。结果:该方法精密度、稳定性和重复性好,平均回收率为94.88%。结论:方法操作简便,专属性强,结果准确,可用于茯茶中的黄酮类成分的监测。

关键词: 茯茶, 高效液相色谱, 黄酮类

Abstract:

An HPLC method for the determination of flavonoids in Fuzhuan tea was presented. Chromatographic separation
was performed on Welchrom C18 (250 mm × 4.6 mm, 5 μm) column by graditent elution with methanol as mobile phase A and
0.5% acetic acid as mobile phase B at a flow rate of 0.8 mL/min. Detection wavelength was 295 nm. It took 80 min to complete
the analytical process. The established method was precise, stable and repeatable, with an average recovery of 94.88%. Thanks
to its ease of use, specificity and accuracy, this method can be used for the determination of flavonoids of Fuzhuan tea.

Key words: Fuzhuan tea, high performance liquid chromatography (HPLC), flavonoid