食品科学 ›› 2013, Vol. 34 ›› Issue (21): 329-332.doi: 10.7506/spkx1002-6630-201321066

• 营养卫生 • 上一篇    下一篇

杏鲍菇发酵产物冻干粉功能成分分析及抗氧化活性

侯金鑫1,江一帆2,赵晓静1,段硕楠1,王立安1,*   

  1. 1.河北师范大学生命科学学院,河北 石家庄 050024;2.华北制药新药研究开发有限公司,河北 石家庄 050015
  • 收稿日期:2013-04-26 修回日期:2013-09-13 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 王立安 E-mail:wlian1965@126.com
  • 基金资助:

    河北省科学技术研究与发展计划项目(11230908D);河北省高等学校科学技术研究重点项目(ZH2011231)

Analysis of Functional Components and Antioxidant Activity of Lyophilized Powder of Cultured Mycelia of Pleurotus eryngii

HOU Jin-xin1,JIANG Yi-fan2,ZHAO Xiao-jing1,DUAN Shuo-nan1,WANG Li-an1,*   

  1. 1. College of Life Science, Hebei Normal University, Shijiazhuang 050024, China;
    2. NCPC New Drug Research and Development Co. Ltd., Shijiazhuang 050015, China
  • Received:2013-04-26 Revised:2013-09-13 Online:2013-11-15 Published:2013-10-28
  • Contact: WANG Li-An E-mail:wlian1965@126.com

摘要:

通过低温冷冻干燥处理将杏鲍菇液体发酵产物制成冻干粉,对冻干粉中的粗纤维、粗多糖、总黄酮和粗三萜功能性成分及其抗氧化活性进行分析。结果表明,冻干粉中粗纤维的含量为52.67mg/g,粗多糖的含量为31.79mg/g,总黄酮的含量为9.19mg/g,粗三萜的含量为8.87mg/g。杏鲍菇发酵产物冻干粉对DPPH自由基的半数有效质量浓度EC50(7.60mg/mL),其质量浓度为16mg/mL时与质量浓度为0.02mg/mL的VC对自由基的清除率相当。抗衰老小鼠实验显示,冻干粉可有效提高小鼠血清、肝脏中的总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平,降低丙二醛(MDA)含量(P<0.05)。杏鲍菇液体发酵产物含有丰富的抗氧化功能性物质,且有较好的开发利用价值。

关键词: 杏鲍菇, 发酵产物, 冻干粉, 总黄酮, 粗三萜, 抗氧化, 衰老小鼠模型

Abstract:

The cultured mycelia of Pleurotus eryngii were lyophilized into powder and the contents of crude fiber, crude
polysaccharide, flavonoids and crude triterpenoid in the lyophilized powder were analyzed. The antioxidant activity was
assessed by using DPPH free radical scavenging assay and D-galactose induced aging mice model. Results showed that
1 g of this powder contained 52.67 mg of crude fiber, 31.79 mg of crude polysaccharides, 9.19 mg of flavonoids and 8.87 mg
of crude triterpenoids. Its EC50 value in quenching DPPH free radical was 7.60 mg/mL. The DPPH radical scavenging
activity of the lyophilized powder at 16 mg/mL was equivalent to that of VC at 0.02 mg/mL. The lyophilized powder was
capable of improving the activities of superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), reducing the
malondiadehyde (MDA) content in serum and liver of aging mice. The cultured mycelia of Pleurotus eryngii could be a
potential source for development and utilization of natural antioxidants.

Key words: Pleurotus eryngii, cultured mycelia, lyophilized powder, total flavonoids, crude triterpenes, antioxidant, aging mice model

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