固相萃取-高效液相色谱法检测肉鸭表皮组织中的松香酸

doi:10.7506/spkx1002-6630-201404017

食品科学 ›› 2014, Vol. 35 ›› Issue (4): 82-85.doi: 10.7506/spkx1002-6630-201404017

固相萃取-高效液相色谱法检测肉鸭表皮组织中的松香酸

张苏珍1,2，耿志明1，王道营1，诸永志1，刘芳1，张牧焓1，卞欢1，徐为民1,*

1.江苏省农业科学院农产品加工研究所，江苏南京 210014；
2.南京农业大学肉品加工与质量控制教育部重点实验室，江苏南京 210095

收稿日期:2013-04-27 修回日期:2014-01-22 出版日期:2014-02-25 发布日期:2014-03-17
通讯作者: 徐为民 E-mail:weiminxu2002@aliyun.com
基金资助:
国家自然科学基金青年科学基金项目（31101312）；江苏省农业科技自主创新资金项目（CX（12）3082）

Determination of Abietic Acid in Duck Skin Tissue by Solid Phase Extraction-High Performance Liquid Chromatography

ZHANG Su-zhen1,2, GENG Zhi-ming1, WANG Dao-ying1, ZHU Yong-zhi1, LIU Fang1, ZHANG Mu-han1, BIAN Huan1, XU Wei-min1,*

1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University,
Nanjing 210095, China

Received:2013-04-27 Revised:2014-01-22 Online:2014-02-25 Published:2014-03-17
Contact: XU Wei-min E-mail:weiminxu2002@aliyun.com

摘要/Abstract

摘要：

采用高效液相色谱法对肉鸭表皮组织中的松香酸含量进行检测。肉鸭表皮组织中的松香酸用乙腈提取，经C18固相萃取柱净化，采用高效液相色谱-紫外法进行检测。色谱柱：反相C18柱（250 mm×4.6 mm，5 μm），流动相：0.003 mol/L磷酸溶液-甲醇（8∶92，v/v）；流速：1 mL/min；检测波长：240 nm。结果表明：方法线性范围为0.1～10 mg/L，检出限为0.10 μg/g，定量限为0.33 μg/g。在0.5～50 μg/g的添加范围内回收率为82.2%～88.0%。实际样品分析结果显示，松香脱毛处理的肉鸭表皮中松香酸含量高达14.52 μg/g。本实验建立的分析方法简便快捷、具有较好的灵敏度和可靠性，可用于肉鸭表皮组织中松香酸的定量分析。

关键词: 肉鸭, 松香, 松香酸, 高效液相色谱法, 固相萃取

Abstract:

A high-performance liquid chromatographic (HPLC) method was established to determine abietic acid in duck
skin. Abietic acid in duck skin samples was extracted with acetonitrile, followed by purification with a C18 solid-phase
extraction cartridge, and determined by HPLC with an ultraviolet (UV) detector. The chromatographic conditions were
established using a C18 column (250 mm× 4.6 mm, 5 μm) with a mobile phase consisting of 0.003 mol/L phosphoric acidmethanol
(8:92, V/V) at a flow rate of 1.0 mL/min and the detection wavelength was set at 240 nm. Results indicated that
the linearity ranged from 0.1 to 10.0 mg/L, the detection limit of abietic acid was 0.1 μg/g, and the quantification limit was 0.33 μg/g.
The recoveries from spiked samples at concentration levels of 0.5–50 μg/g were in the range of 82.2%–88.0%. The
developed method was successfully applied to the determination of abietic acid in duck samples epilated with colophony.
The content of abietic acid in the duck sample could reach 14.52 μg/g. This method proved to be of high maneuverability as
well as excellent sensitivity and accuracy, and could be used for quantitative analysis of abietic acid in duck skin tissue.

Key words: duck, rosin, abietic acid, high performance liquid chromatography (HPLC), solid phase extraction (SPE)

中图分类号:

TS207
O657

张苏珍1,2，耿志明1，王道营1，诸永志1，刘芳1，张牧焓1，卞欢1，徐为民1,*. 固相萃取-高效液相色谱法检测肉鸭表皮组织中的松香酸[J]. 食品科学, 2014, 35(4): 82-85.

ZHANG Su-zhen1,2, GENG Zhi-ming1, WANG Dao-ying1, ZHU Yong-zhi1, LIU Fang1, ZHANG Mu-han1, BIAN Huan1, XU Wei-min1,*. Determination of Abietic Acid in Duck Skin Tissue by Solid Phase Extraction-High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2014, 35(4): 82-85.

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固相萃取-高效液相色谱法检测肉鸭表皮组织中的松香酸

Determination of Abietic Acid in Duck Skin Tissue by Solid Phase Extraction-High Performance Liquid Chromatography

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