食品科学 ›› 2014, Vol. 35 ›› Issue (2): 126-131.doi: 10.7506/spkx1002-6630-201402023

• 工艺技术 • 上一篇    下一篇

高良姜多糖提取工艺优化及其抗氧化活性研究

郑 义1,2,王卫东1,2,李 勇1,2,朱园园1,郭 静1   

  1. 1.徐州工程学院食品(生物)工程学院,江苏 徐州 221000;
    2.江苏省食品资源开发与质量安全重点建设实验室,江苏 徐州 221000
  • 收稿日期:2013-04-28 修回日期:2013-12-19 出版日期:2014-01-25 发布日期:2014-02-19
  • 通讯作者: 郑义 E-mail:biozheng@gmail.com
  • 基金资助:

    苏北科技发展计划项目(BC2011401)

Optimization of Extraction Process and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance

ZHENG Yi1,2, WANG Wei-dong1,2, LI Yong1,2, ZHU Yuan-yuan1, GUO Jing1   

  1. 1. College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China;
    2. Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou 221000, China
  • Received:2013-04-28 Revised:2013-12-19 Online:2014-01-25 Published:2014-02-19
  • Contact: ZHENG Yi E-mail:biozheng@gmail.com

摘要:

通过Box-Behnken试验设计,获得了热水浸提高良姜多糖的最佳工艺;以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力、还原力、清除羟自由基能力、螯合铁离子能力为指标,评价了高良姜多糖的抗氧化活性。结果表明,热水浸提高良姜多糖的最佳工艺条件为液料比43∶1(mL/g)、浸提温度95 ℃、浸提时间3 h,在此条件下多糖得率实测值为11.81%。高良姜多糖具有较好的抗氧化活性,清除自由基能力、还原力和螯合铁离子能力均表现出一定的质量浓度依赖性;高良姜多糖清除DPPH自由基、清除羟自由基和螯合铁离子能力的半数有效质量浓度(EC50)分别为(0.59±0.01)、(0.05±0.003)g/L和(2.75±0.2)g/L。

关键词: 高良姜, 多糖, 响应面法, 抗氧化活性

Abstract:

The conditions for hot water extraction of polysaccharides from Alpinia officinarum Hance were optimized by
Box-Behnken statistical design. The antioxidant activities of polysaccharides extracted from Alpinia officinarum Hance
were investigated using four different in vitro antioxidant assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging
activity, reducing power, hydroxyl radical scavenging activity and iron-chelating ability. The results showed that the optimal
extraction conditions were found to be extraction at 95 ℃ for 3 h with a solvent-to-solid ratio of 43:1 (mL/g). The experimentally
observed yield of polysaccharides extracted from Alpinia officinarum Hance was 11.81% under these conditions. The
extracted polysaccharides had powerful antioxidant activities for free radical scavenging activity, reducing power and ironchelating
capacity in concentration-dependent manner, with median effective concentration (EC50) of (0.59 ± 0.01), (0.05 ± 0.003)
and (2.75 ± 0.2) g/L, respectively.

Key words: Alpinia officinarum Hance, polysaccharides, response surface methodology, antioxidant activity

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