海藻酸钠-培养基-刃天青-硅藻土微球法快速检测细菌总数

doi:10.7506/spkx1002-6630-201404037

食品科学 ›› 2014, Vol. 35 ›› Issue (4): 179-185.doi: 10.7506/spkx1002-6630-201404037

海藻酸钠-培养基-刃天青-硅藻土微球法快速检测细菌总数

韦伟1,2，李正国2，程婵娟3，杨德芳4

1.重庆师范大学生命科学学院，重庆 401331；2.重庆大学生命科学学院基因工程研究中心，重庆 400045；
3.重庆市第95中学，重庆 400084；4.重庆市巴蜀中学，重庆 400013

收稿日期:2013-05-08 修回日期:2014-01-18 出版日期:2014-02-25 发布日期:2014-03-17
通讯作者: 韦伟 E-mail:weiwei_cqnu@163.com
基金资助:
重庆师范大学精品课程项目（重师教发[2010]No.138）；重庆师范大学校级青年基金项目（12XWQ15）；
重庆师范大学“活性物质生物技术教育部工程研究中心”建设项目（GCZX2012-4）

Rapid Detection of Total Bacterial Number Using Alginate-Medium-Resazurin-Diatomaceous Silica Microspheres Method

WEI Wei1,2, LI Zheng-guo2, CHENG Chan-juan3, YANG De-fang4

1. College of Life Science, Chongqing Normal University, Chongqing 400047, China; 2. Genetic Engineering Research Center,
College of Bio-Engineering, Chongqing University, Chongqing 400045, China; 3. Chongqing No. 95 Middle School,
Chongqing 400084, China; 4. Chongqing Bashu Middle School, Chongqing 400013, China

Received:2013-05-08 Revised:2014-01-18 Online:2014-02-25 Published:2014-03-17
Contact: Wei WEI E-mail:weiwei_cqnu@163.com

摘要/Abstract

摘要：

通过制备海藻酸钠-培养基-刃天青-硅藻土（alginate-medium-resazurin-diatomaceous silica，AMRD）微球，建立一种快速检测细菌总数的新方法。结果表明：单倍培养基浓度下，海藻酸钠∶硅藻土为1∶1.4（m/m）时，AMRD微球变色时间与细菌数量呈最佳线性关系，检测限为2.02×102 CFU/mL，检测时间为2～16.5 h。AMRD微球对大肠杆菌和金黄色葡萄球菌的检测灵敏度分别为1.62×103、2.03×103 CFU/mL。微球对细菌的吸附符合Freundlich等温吸附方程，参数1/n在0.5～1.0 之间，属于中等吸附，低浓度条件下受温度影响不大，吸附饱和需要30～45 min。AMRD微球细菌计数法是一种快速而经济的细菌计数新方法，有望用于食品、饮用水以及环境检测等领域细菌总数的定量检测。

关键词: 海藻酸钠, 硅藻土, 刃天青, 细菌计数, 细菌吸附

Abstract:

Alginate-medium-resazurin-diatomaceous silica microspheres (AMRD microspheres) were prepared to establish
a rapid method for counting the number of bacterial cells. The results suggested that the positive optimum linear correlation
between the concentration of bacterial cells and discoloration time of AMRD microcapsules was achieved at a ratio of
alginate to diatomaceous silica of 1:1.4 with single medium concentration. The limit of detection (LOD) of the method was
2.02×102 CFU/mL. The detection time was 2–16.5 h. The detection sensitivities for Escherichia coli and Staphylococcus
aureus were 1.62 × 103 CFU/mL and 2.03 × 103 CFU/mL, respectively. The adsorption isotherms could be described
by the Freundlich equation and the coefficient of adsorption (1/n) was 0.5–1.0, which was thus considered as moderate
adsorption. No significant change in the adsorption capacity was observed at varying temperatures at a low bacterial cell
concentration and the time needed to reach the maximum bacterial adsorption balance was 30–45 min. In conclusion, AMRD
microcapsules is a new rapid and economical bacterial cell counting method, which can be applied in the fields of food,
water source and environmental monitoring.

Key words: alginate, diatomaceous silica, resazurin, bacterial cell counting, adsorption for bacteria

中图分类号:

Q93.332

韦伟1,2，李正国2，程婵娟3，杨德芳4. 海藻酸钠-培养基-刃天青-硅藻土微球法快速检测细菌总数[J]. 食品科学, 2014, 35(4): 179-185.

WEI Wei1,2, LI Zheng-guo2, CHENG Chan-juan3, YANG De-fang4. Rapid Detection of Total Bacterial Number Using Alginate-Medium-Resazurin-Diatomaceous Silica Microspheres Method[J]. FOOD SCIENCE, 2014, 35(4): 179-185.

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海藻酸钠-培养基-刃天青-硅藻土微球法快速检测细菌总数

Rapid Detection of Total Bacterial Number Using Alginate-Medium-Resazurin-Diatomaceous Silica Microspheres Method

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