食品科学 ›› 2014, Vol. 35 ›› Issue (4): 44-49.doi: 10.7506/spkx1002-6630-201404010

• 工艺技术 • 上一篇    下一篇

响应面优化超声波提取猕猴桃根熊果酸工艺

杨 艳,任亚梅*,马 婷,周九庆,查 晶,张哲学   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 收稿日期:2013-02-18 修回日期:2014-01-07 出版日期:2014-02-25 发布日期:2014-03-17
  • 通讯作者: 任亚梅 E-mail:yameiren@yahoo.com
  • 基金资助:

    西北农林科技大学科技创新与成果转化项目(Z222021313)

Optimization of Ultrasonic-Assisted Extraction of Ursolic Acid from Roots of Actinidia chinensis Using Response Surface Analysis

YANG Yan, REN Ya-mei*, MA Ting, ZHOU Jiu-qing, ZHA Jing, ZHANG Zhe-xue   

  1. College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling 712100, China
  • Received:2013-02-18 Revised:2014-01-07 Online:2014-02-25 Published:2014-03-17
  • Contact: ren yamei E-mail:yameiren@yahoo.com

摘要:

为优化猕猴桃根熊果酸的超声波提取最佳工艺,在单因素试验基础上,选择乙醇体积分数、液料比、提取时间、提取温度为自变量,熊果酸得率为响应值,利用中心组合试验法和响应面分析法,研究各自变量交互作用及其对熊果酸得率的影响,模拟得到二次多项式回归方程的预测模型。结果表明:当超声功率225 W、超声频率40 kHz时,超声波提取熊果酸工艺最佳条件为乙醇体积分数73.8%、液料比7.80∶1(mL/g)、提取时间23.2 min、提取温度79.1 ℃。此提取条件下,熊果酸得率达到2.014%,与模型预测值之间具有较好的拟合性,且提取液中熊果酸的纯度为49.7%。

关键词: 猕猴桃根, 熊果酸, 超声波提取, 响应面分析

Abstract:

In this study, the conditions for ultrasonic-assisted extraction of ursolic acid (UA) from the roots of Actinidia
chinensis were optimized by response surface methodology. On the basis of single-factor experiments, a mathematical
regression model was established indicating the effect of ethanol concentration, solvent/solid ratio, extraction time and
temperature on the extraction yield of UA by response surface central composite design. Under the conditions of 225 W and
40 kHz, the optimum extraction conditions determined were as follows: 73.8% ethanol concentration, 7.80:1 solvent/solid
ratio (mL/g), 23.2 min and 79.1 ℃. Under these conditions, the experimental extraction yield of UA was 2.014%, which is
well matched with the predicted value, and the purity of UA in the extract was 49.7%.

Key words: Actinidia chinensis root, ursolic acid, ultrasonic-assisted extraction, response surface analysis

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