食品科学 ›› 2014, Vol. 35 ›› Issue (2): 47-50.doi: 10.7506/spkx1002-6630-201402009

• 工艺技术 • 上一篇    下一篇

高速逆流色谱分离纯化苦荞中芦丁、槲皮素

朱 琳,任 清*,徐笑颖   

  1. 北京工商大学食品学院,食品添加剂与配料北京高校工程研究中心,北京 100048
  • 收稿日期:2013-05-17 修回日期:2013-12-24 出版日期:2014-01-25 发布日期:2014-02-19
  • 通讯作者: 任清 E-mail:renqing@th.btbu.edu.cn
  • 基金资助:

    国家现代农业产业技术体系建设专项(CARS-08-D-3)

Isolation and Preparation of Flavones from the Bran of Tartary Buckwheat Using High Speed Counter Current Chromatography

ZHU Lin, REN Qing*, XU Xiao-ying   

  1. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering,
    Beijing Technology and Business University, Beijing 100048, China
  • Received:2013-05-17 Revised:2013-12-24 Online:2014-01-25 Published:2014-02-19
  • Contact: REN Qing E-mail:renqing@th.btbu.edu.cn

摘要:

利用乙醇溶液提取苦荞麸皮中总黄酮,将得到的苦荞黄酮粗提物用DA-201大孔树脂进行初步纯化。以 得到的黄酮初步纯化产物为原料,应用高速逆流色谱对其中的芦丁和槲皮素进行分离、纯化。所用两相溶剂体系 为乙酸乙酯-正丁醇(4∶1∶5,v/v),上相为固定相,下相为流动相,流速2 mL/min,转速850 r/min,检测波长 254 nm。所得产物经高效液相色谱检测,结果表明得到芦丁纯度为99%,槲皮素纯度为94%。表明该法分离制备苦 荞麸皮中芦丁、槲皮素简便,所得产物纯度高。

关键词: 苦荞, 芦丁, 槲皮素, 高速逆流色谱

Abstract:

The bran of tartary buckwheat was extracted with 60% ethanol. A DA-201 macroporous resin column was applied
in the purification of flavonoids from the extract. High speed counter current chromatography (HSCCC) method was used
for preparative isolation and purification of rutin and quercetin from the preliminarily purified extracts. A two-phase solvent
system composed of ethyl acetate, n-butyl alcohol and water (4:1:5, V/V) was used at a flow rate of 2.0 mL/min for HSCCC
separation at a rotation speed of 850 r/min and the pooled eluates were detected at 254 nm. The purity of rutin and quercetin
obtained was 99% and 94%, respectively, as detected by high performance liquid chromatography (HPLC). These results
indicate that this method is convenient and effective and can be used to extract and purify rutin and quercetin from tartary
buckwheat bran.

Key words: tartary buckwheat, rutin, quercetin, high speed counter current chromatography (HSCCC)

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